| To study the influence of space environment on the leaf callus of alfalfa seed carried by satellite, the callus of alfalfa under different stress (NaCl and PEG and temperature stress) were studied, and the five physiological parameters including proline, soluble sugar, soluble protein, relative conductivity and MDA content were determined to evaluate the effect of satellite carrying on callus resistance. The results showed that the survival rates of no-carrying material were 2.68%, 3.8%, 43.68%, and 35.42% for NaCl (2%), PEG (30%), high temperature (40℃, 6 d), and low temperature (0℃, 8 d) stress, being significantly different with those of the control. Satellite-carrying resulted in higher proline, soluble sugar, protein contents, by an increase of 55.6%、34.71%, and 40.67%, respectively. By contrast, the relative conductivity and MDA content were reduced by 48.37% and 39.47%. The degree of resistance of satellite carrying alfalfa callus was in the order of drought, salt, low temperature, and high temperature. |