| Cloning and functional analysis of a BdCKX gene in Brachypodium distachyon |
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| DOI：10.7606/j.issn.1000-7601.2016.03.41 |
| Key Words: Brachypodium distachyon CKX overexpression cytokinin |
| Author Name | Affiliation | | LI Jia | College of Agronomy, Northwest A&F University, Yangling, Shaanxi 712100, China | | ZHOU Xue | College of Agronomy, Northwest A&F University, Yangling, Shaanxi 712100, China | | LI Ting-ting | College of Agronomy, Northwest A&F University, Yangling, Shaanxi 712100, China | | WANG Zhong-hua | College of Agronomy, Northwest A&F University, Yangling, Shaanxi 712100, China | | QUAN Li | College of Agronomy, Northwest A&F University, Yangling, Shaanxi 712100, China |
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| Abstract: |
| As one of the most important plant hormones, cytokinin plays diverse roles during plant development including regulation of cell division, promotion of shoot growth etc. The metabolism of cytokinin is primarily dependent on functions of cytokinin oxidase encoding genes. To gain insightful understanding and take full advantage of cytokinin oxidase in the genetic improvement of crop growth traits, a homologous gene to the AtCKX3 (Arabidopsis Cytokinin Oxidase 3) gene in Brachypodium distachyon, BdCKX, was cloned. To elucidate its bioloical function, CDS sequence of the BdCKX gene was amplified and ligated with the pCXSN vector, which was then introduced to callus tissue of young embryo by Agrobacterium-mediated transformation for over-expression analysis. Positive transformants exhibited phenotypes including short stem, shrunk leaf, retarded development, and small overall shoot. It could be concluded that the elevated expression of BdCKX gene was able to interfere plant growth through the degradation of endogenous cytokinin levels. In addition, gene expression profile indicated that BdCKX was differentially expressed in various tissues at different developmental stages. In particular, expression level of BdCKX was markedly shifted in leaf of 40 days old seedling. Because cytokinin encoding genes were conserved among Pooideae species, our research may provide a theoretical base for the regulatory mechanism and genetic improvement of Triticeae crops. |
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