| The in vitro effects of mannitol and uniconazole on cultivation of potato seedlings |
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| DOI：10.7606/j.issn.1000-7601.2016.04.14 |
| Key Words: mannitol uniconazole potato in vitro tissue culturing seedlings inoxidizability |
| Author Name | Affiliation | | WANG Dong-xia | College of Agronomy, Gansu Agriculture University, Lanzhou, Gansu 730070, China | | YANG Hong-yu | College of Horticulture, Gansu Agricultural University, Lanzhou, Gansu 730070, China | | CAO Dong | Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, Qinghai 810008, China |
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| Abstract: |
| To explore the effects of mannitol and uniconazole with different concentrations on healthy potato seedlings by in vitro tissue culturing, a test containing a control group (CK) and treatments group including A (10 g·L-1 Mannitol), B (20 g·L-1 Mannitol), C (30 g·L-1 Mannitol), D (40 g·L-1 Mannitol), E (0.001 mg·L-1 Uniconazole), F (0.002 mg·L-1 Uniconazole), G (0.003 mg·L-1 Uniconazole), and H (0.004 mg·L-1 Uniconazole) was carried out by repeating three times for every level. Samples were measured at the 30th and 60th days, respectively. The results indicated that after 30 days and 60 days with appropriate Mannitol (20 g·L-1) and Uniconazole (0.003 mg·L-1) treatments, potato seedlings were dwarf (Plant height: 82.54±3.84 mm and 67.41±1.67 mm at the 30th day; 93.74±1.30 mm and 68.78±1.16 mm at the 60th day) and thickened (Stem diameter: 1.45±0.03 mm and 1.32±0.05 mm at the 30 th day; 1.80±0.13 mm and 1.65±0.03 mm at the 60th day). Also, fresh weight of potato reached to the maximum (Fresh weight per plant: 1.35±0.04 g and 1.32±0.04 g at the 30th day; 1.75±0.09 g and 1.53±0.04 g at the 60th day). In addition, seedlings root length, diameter, surface area and volumes were also significantly higher than CK. Meanwhile, the enzymatic activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) were increased and reached the maximum under these treatments. Therefore, Mannitol and Uniconazole were beneficial for thickening and dwarfing of potato seedlings, root system enhancement, and increased antioxidant enzyme activities and decrease in damage of potato seedlings by active oxygen, which is conducive in cultivating strong seedlings. |
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