| Screening of Trichoderma sp. strain for high phosphorus solubilization using microwave mutagenesis |
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| DOI：10.7606/j.issn.1000-7601.2016.04.35 |
| Key Words: Trichoderma sp. microwave mutagenesis phosphate solubilization capacity antagonistic effect |
| Author Name | Affiliation | | XUE Ying-yu | College of Plant Protection, Gansu Agricultural University, Lanzhou, Gansu 730030, China | | YE Wei | College of Plant Protection, Gansu Agricultural University, Lanzhou, Gansu 730030, China | | ZHANG Shu-wu | College of Plant Protection, Gansu Agricultural University, Lanzhou, Gansu 730030, China | | LIU Jia | College of Plant Protection, Gansu Agricultural University, Lanzhou, Gansu 730030, China | | XU Bing-liang | College of Plant Protection, Gansu Agricultural University, Lanzhou, Gansu 730030, China |
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| Abstract: |
| In order to obtain Trichoderma sp. strain with high phosphorus dissolving efficiency, the original strain, Trichoderma T6 with low ability in dissolving phosphorus, was mutated by microwave. The microwave mutagenesis parameters were optimized, and the genetic stability, phosphate solubilizing power and antibiotic ability of the mutant were investigated. The results showed that the optimum mutagenic method was with an irradiation power at 900 W and a mutation time of 70 s. 8 stains with high phosphorus solubilizing capability named T6-9, T6-33, T6-93, T6-126, T6-157, T6-188, T6-196 and T6-203 were obtained using the optimized mutagenic method. After several generations, a strain named T6-157 with relatively stable hereditary was eventually obtained, exhibiting high phosphorus solubilizing capability. The highest phosphate solubilizing power of mutant strain T6-157 was 204.46 mg·L-1, and the phosphate solubilizing power and phytase activity were increased by 107.97% and 57.35%, respectively, compared with the original strain. The antagonistic ability against the main soil-borne pathogens was higher than that of the initial strain T6. |
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