Comprehensive evaluation of drought resistance of different Medicago ruthenica germplasms at the budding stage
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DOI:10.7606/j.issn.1000-7601.2016.05.09
Key Words: Medicago ruthenica  budding stage  drought resistance  subordinate function
Author NameAffiliation
XIAO Hong Pratacultural College, Gansu Agricultural University/Key Laboratory of Grassland Ecosystem of Ministry of Education/ Sino-U.S. Centers for Grazing land Ecosystem Sustainability, Lanzhou, Gansu 730070, China 
WANG Fang Agronomy College, Gansu Agricultural University, Lanzhou, Gansu 730070, China 
DUAN Chun-hua Fodder and Forage Station of Xuanhan County, Xuanhan, Sichuan 636150, China 
JING Yuan-yuan Pratacultural College, Gansu Agricultural University/Key Laboratory of Grassland Ecosystem of Ministry of Education/ Sino-U.S. Centers for Grazing land Ecosystem Sustainability, Lanzhou, Gansu 730070, China 
CHEN Lu-jun Pratacultural College, Gansu Agricultural University/Key Laboratory of Grassland Ecosystem of Ministry of Education/ Sino-U.S. Centers for Grazing land Ecosystem Sustainability, Lanzhou, Gansu 730070, China 
ZHANG Jian-wen Pratacultural College, Gansu Agricultural University/Key Laboratory of Grassland Ecosystem of Ministry of Education/ Sino-U.S. Centers for Grazing land Ecosystem Sustainability, Lanzhou, Gansu 730070, China 
YANG Hai-lei Pratacultural College, Gansu Agricultural University/Key Laboratory of Grassland Ecosystem of Ministry of Education/ Sino-U.S. Centers for Grazing land Ecosystem Sustainability, Lanzhou, Gansu 730070, China 
YU Xiao-jun Pratacultural College, Gansu Agricultural University/Key Laboratory of Grassland Ecosystem of Ministry of Education/ Sino-U.S. Centers for Grazing land Ecosystem Sustainability, Lanzhou, Gansu 730070, China 
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Abstract:
      With optimal, medium and severe water stresses (soil relative water contents were 75%~80%, 50%~55%, and 30%~35%, respectively), the drought resistances of 12 Medicago ruthenica germplasms from different habitats at the budding stage were studied by a pot culturing method by repeated drought stress. The results showed that plant height, stem diameter, aboveground and underground biomass, leaf relative water content and chlorophyll content were all decreased with the increase in severity of drought stress, while the MDA content was increased. Compared with the control (CK), the decreasing levels of leaf relative water content for 12 M. ruthenica germplasms were all below 11% under medium water stress. The growth of M. ruthenica was seriously inhibited under severe water stress. In addition, under medium and severe water stresses, the plant heights of M. ruthenica (Longxian) were declined by 1.8% and 22.1% respectively, and the leaf MDA contents of M. ruthenica (Weiyuan) were 26.172 μmol·g-1 and 31.149 μmol·g-1, respectively, higher than other materials. Based on the relative values of plant height, stem diameter, aboveground biomass, underground biomass, leaf relative water content, MDA content and chlorophyll content under medium and severe water stresses, the drought resistance of 12 Medicago ruthenica germplasms was comprehensive evaluated by the subordinate function method and followed the order from strong to weak as Longxian (from Shanxi)>Yuzhong>Yongchang>Longxi>Tianshui>Weiyuan>Tumote>No.8 Zixuan>Jingtai>Linxia>Zhenyuan>Xiahe.