| Changes of photosynthetic and antioxidant properties of Gardenia jasminoides seedlings treated with 5-aminolevulinic acid and PEG |
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| DOI：10.7606/j.issn.1000-7601.2016.06.36 |
| Key Words: Gardenia jasminoides PEG stress 5-aminolevulinic acid photosynthesis antioxidant property |
| Author Name | Affiliation | | OU Chun | School of Biotechnology and Food Engineering, Fuyang Normal College, Fuyang, Anhui 236037, China | | YAO Xia-mei | School of Biotechnology and Food Engineering, Fuyang Normal College, Fuyang, Anhui 236037, China
State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry Sciences, Bejing 100091, China | | YAO Xiao-jie | Anhui Jianzhu University, Hefei, Anhui 230022, China | | JI Jing | State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry Sciences, Bejing 100091, China | | WANG Wei-gen | School of Biotechnology and Food Engineering, Fuyang Normal College, Fuyang, Anhui 236037, China | | GUO Jian | School of Biotechnology and Food Engineering, Fuyang Normal College, Fuyang, Anhui 236037, China |
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| Abstract: |
| The alleviative effects of exogenous 5-aminolevulinic acid (ALA) on plants against drought stress were assessed in Gardenia jasminoides seedlings treated with ALA at different concentrations. By water culuring, G. jasminoides seedlings were cultured with 15% PEG6000 and ALA with different concentrations (0, 25, 50, 100 mg·L-1 and 200 mg·L-1), and water was used as control. Their physiological and biochemical indexes were studied, including organic osmolytes content, photosynthesis and antioxidant system. The results showed that seedlings exposed to 15% PEG for 14 d exhibited a decrease in aboveground and underground dry mass, seedling height, root length, relative water content, photosynthetic pigment content, net photosynthetic rate (Pn), transpiration rate (Tr), stomatal conductance (Gs), and water use efficiency. In PEG-stressed plants, the levels of proline, malondialdehyde (MDA), hydrogen peroxide (H2O2), and electrolyte leakage were significantly increased, whereas antioxidative activity, including superoxide, peroxidase, and catalase activities, went declined in leaves. However, the presence of ALA provided an effective method of mitigating PEG-caused physiological stresses on G. jasminoides seedlings, which depended on ALA levels. PEG-treated plants exposed to ALA at 50 mg·L-1 significantly eased PEG-induced growth inhibition. Application of ALA, especially at the concentration of 50 mg·L-1, considerably raised chlorophyll a content, chlorophyll b content, Pn, SOD, POD, CAT activity, relative water content and proline accumulation by 25.35%, 31.85%, 228.68%, 41.92%, 27.26%, 41.50%, 170.26%, and 31.01%, respectively, while decreased MDA content, H2O2 content, and electrolyte leakage by 39.00%, 31.18%, and 45.56%, respectively. By contrast, the positive effects were not evident, or even more severe in the PEG+200 mg·L-1 ALA treatment. |
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