Effects of seed film coating with exogenous regulating substances on cotton germination rate and cold tolerance of seedlings
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DOI:10.7606/j.issn.1000-7601.2017.01.29
Key Words: cotton  germination rate  film coating with exogenous regulating substances  low temperature stress  cold resistance of seedlings
Author NameAffiliation
LI Fang-zhou The Key Laboratory of Oasis Eco-agriculture of Xinjiang Production Construction Group/College of Agriculture, Shihezi University, Shihezi, Xinjiang 832003, China 
YE Jun The Key Laboratory of Oasis Eco-agriculture of Xinjiang Production Construction Group/College of Agriculture, Shihezi University, Shihezi, Xinjiang 832003, China 
HOU Zhen-an The Key Laboratory of Oasis Eco-agriculture of Xinjiang Production Construction Group/College of Agriculture, Shihezi University, Shihezi, Xinjiang 832003, China 
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Abstract:
      The aim of this experiment was to study the effects of seed film coating with exogenous regulating substances on the cotton seed germination and physiological response of cold tolerance in cotton seedlings under low temperature stress of 5℃ by sand culture method. The results showed that the low temperature stress inhibited the cotton seed germination and growth of seedlings. Seeds coated with salicylic acid and exogenous regulator compound had better germination potential, and higher germination rate, germination index and vigor index. Compared with CK, seeds coated with exogenous regulator compound had germination index increased by 41.2%~44.4%, vigor index 30.9%~63.9%. The coating treatments with exogenous regulating substances decreased leaf REC and MDA content. It also increased sharply the soluble protein and soluble sugar contents. The activity of SOD, POD and CAT were significantly increased. The coating treatment with a compound preparation of 5 mmol·L-1 salicylic acid, 45 mmol·L-1 calcium chloride and 60 mg·L-1 sodium selenite which REC and MDA content were significantly higher than other coating treatment, and soluble protein content were significantly higher than other coating treatment. These results demonstrated that seed film coating with salicylic acid, calcium chloride and sodium selenite has a synergistic effect of improving low temperature resistance of cotton seedling by promoting seed germination and seedling growth.