| QTL analysis of cold tolerance for physiological traits of winter rapeseed in northern arid-cold area |
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| DOI：10.7606/j.issn.1000-7601.2017.01.31 |
| Key Words: winter rapeseed (Brassica rapa L.) cold resistance QTL northern arid-cold area |
| Author Name | Affiliation | | WU Jun-yan | College of Agronomy, Gansu Agricultural University, Gansu Key Lab of Crop Improvement and Germplasm Enhancement, Gansu Provincial Key Laboratory of Aridland Crop Science, Lanzhou, Gansu 730070, China | | KONG De-jing | College of Agronomy, Gansu Agricultural University, Gansu Key Lab of Crop Improvement and Germplasm Enhancement, Gansu Provincial Key Laboratory of Aridland Crop Science, Lanzhou, Gansu 730070, China | | FANG Yan | College of Agronomy, Gansu Agricultural University, Gansu Key Lab of Crop Improvement and Germplasm Enhancement, Gansu Provincial Key Laboratory of Aridland Crop Science, Lanzhou, Gansu 730070, China | | SUN Wan-cang | College of Agronomy, Gansu Agricultural University, Gansu Key Lab of Crop Improvement and Germplasm Enhancement, Gansu Provincial Key Laboratory of Aridland Crop Science, Lanzhou, Gansu 730070, China | | LIU Hai-qing | College of Agronomy, Gansu Agricultural University, Gansu Key Lab of Crop Improvement and Germplasm Enhancement, Gansu Provincial Key Laboratory of Aridland Crop Science, Lanzhou, Gansu 730070, China | | LIU Zi-gang | College of Agronomy, Gansu Agricultural University, Gansu Key Lab of Crop Improvement and Germplasm Enhancement, Gansu Provincial Key Laboratory of Aridland Crop Science, Lanzhou, Gansu 730070, China | | LI Xue-cai | College of Agronomy, Gansu Agricultural University, Gansu Key Lab of Crop Improvement and Germplasm Enhancement, Gansu Provincial Key Laboratory of Aridland Crop Science, Lanzhou, Gansu 730070, China |
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| Abstract: |
| The superior cold resistance B. rapa variety “Longyou 7” and general cold variety “Longyou 9” were used as parents and a F2 mapping population was constructed. The results showed that 39 primers were selected for further analysis of F2 population. Based on molecular marker genetic map and QTL software, a total of 24 QTLs were detected for SOD activity, POD activity, CAT activity, MDA content, free proline content and soluble protein content from 10 chromosomes. Those QTLs were able to explain 11.1783%～81.1753% of phenotypic variation. The dominant effects of 10 QTLs were positive effects, LOD values were between 3.2787～163.7958. The data showed that the regions BrID90127-BrID10421，BrID10421-BrID10709 and BrID10709-BrID101165 on 2A, BRMS034(R5)-Ra3-H10 on 5A,Ra1-F06-Ra2-D04 on 6A, BrID10839-Ra2-E12 on 8A, BrID90115-Ra2-E07 on 10A were common regions shared by multi-traits. The result of present study is useful for the accurate position of winter rapa. |
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