Physiological responses and tolerance of three maize inbred lines to salt stress |
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DOI:10.7606/j.issn.1000-7601.2017.02.15 |
Key Words: maize salt subordinate function |
Author Name | Affiliation | WANG Jing-ze | College of Agronomy, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China | GAO Shu-ren | College of Agronomy, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China | SUN Li-fang | College of Agronomy, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China | WANG Xia | College of Agronomy, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China | WANG Jing | College of Agronomy, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China | HU Kai-feng | College of Agronomy, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China | DENG Jie | College of Agronomy, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China |
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Abstract: |
Using three maize inbred lines (including M-2 introgression line, Hr30 introgression line, and Mo17 introgression line) as test materials, an experiment was conducted to investigate the effects of different concentrations of NaCl(0 mmol·L-1, 100 mmol·L-1, 150 mmol·L-1, 200 mmol·L-1, and 250 mmol·L-1)on physiological parameters such as superoxide dismutase (SOD) activity, peroxidase (POD) activity and malondialdehyde (MDA) content in maize seedlings. Furthermore, the salt tolerance of these three inbred lines was comprehensively evaluated by the methods of principal components analysis and fuzzy subordinate function, so as to lay foundation of identifying salt tolerance and selecting salt-tolerant cultivars of maize. The results showed that, with the increase of salt concentration, the plant height, biomass, chlorophyll content, net photosynthetic rate (Pn) and soluble protein content in all three inbred lines tended to decrease, while the root length, relative conductivity, MDA content, SOD activity, POD activity and root/shoot ratio were increased at first and decreased subsequently. According to the results of principal components analysis, 9 parameters (including plant height, Pn, MDA content, SOD activity and so on) were closely related to salt tolerance. Through the analysis of fuzzy subordinate function, the salt-resistant capability of tested inbred lines was ranked as: M-2 introgression line>Hr30 introgression line>Mo17 introgression line. |
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