| Effects of exogenous ABA and Ca2+ on sucrose metabolism in grape (Vitisvinifera L.) seedlings under low temperature treatment and recovery |
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| DOI：10.7606/j.issn.1000-7601.2017.02.20 |
| Key Words: Vitisvinifera L. ABA Ca2+ low temperature sucrose metabolism |
| Author Name | Affiliation | | JIANG Han-yu | College of Life Science and Technology, Gansu Agricultural University, Lanzhou, Gansu 730070, China | | WANG Wang-tian | College of Life Science and Technology, Gansu Agricultural University, Lanzhou, Gansu 730070, China | | LEI Tian-xiang | Northwest Plateau Institute of Biology, Chinese Academy of Science, Xining, Qinghai 810008, China | | HE Bai-jun | Gansu Zixuan Wine Co., Ltd., Jiayuguan, Gansu 735100, China | | ZHANG Jin-lin | College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, Gansu 730020, China |
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| Abstract: |
| In order to figure out the responsing mechanisms of exogenous abscisic acid (ABA) and Ca2+ on sucrose metabolism and key enzymes under low temperature, we measured the contents of sucrose, glucose, fructose and starch, the activities of sucrose-phosphate synthase (SPS), sucrose synthase (SS), acid invertase (AI) and neutral invertase (NI) in Cabernets (Vitisvinifera L.) leaves at 5℃ and recovery at 25℃ treatment. The results showed with the increase in concentrations of ABA or Ca2+, the contents of sucrose, glucose, fructose and starch were increased significantly, and they were at the maximums when the concentrations of ABA or Ca2+ were 5 mg·L-1 or 15 mmol·L-1, resulting in increases of 67.88%, 21.8%, 213.77%, 7.04%, 51.38%, 2.35%, 8.84%, and 31.81%, or 72.81%, 35.37%, 123.57%, 36.44%, 25.90%, 28.67%, 210.56%, and 210.90%, respectively; The activities of acid invertase (AI), neutral invertase (NI), sucrose-phosphate synthase (SPS) and sucrose synthase (SS) were also increased markedly by 64.10%, 125.00%, 193.29%, 756.31%, 2 291.51%, 1 569.31%, 1 523.13%, and 1 336.77%, or 26.42%, 20.38%, 30.75%, 58.30%, 111.63%, 14.14%, 14.32%, and 66.39%, respectively. Our results provided evidence that exogenous ABA or Ca2+ may impose damages to grape seedlings caused by low temperature. Key components of the response are: enhanced the activities of AI, NI, SS and SPS, increased soluble sugar level in leaves, promoting cold resistance ability for seedling. |
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