| A comprehensive evaluation of salt-tolerance and physiological response of transgenic potato to salt stress in field by the introduction of AtNHX1 gene |
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| DOI：10.7606/j.issn.1000-7601.2017.03.21 |
| Key Words: potato AtNHX1 gene salt stress physiological parameter comprehensive evaluation of salt tolerance |
| Author Name | Affiliation | | LI Li-xia | Gansu Key Laboratory of Crop Genetic and Germplasm Enhancement/College of Horticulture/Gansu Key Laboratory of Aridland Crop Science, Gansu, Lanzhou 730070, China | | LIU Yu-hui | Gansu Key Laboratory of Crop Genetic and Germplasm Enhancement/College of Horticulture/Gansu Key Laboratory of Aridland Crop Science, Gansu, Lanzhou 730070, China | | WANG Li | College of Life Sciences and Technology, Gansu Agricultural University, Gansu, Lanzhou 730070, China | | YU Bin | Gansu Key Laboratory of Crop Genetic and Germplasm Enhancement/College of Horticulture/Gansu Key Laboratory of Aridland Crop Science, Gansu, Lanzhou 730070, China | | ZHANG Jun-lian | Gansu Key Laboratory of Crop Genetic and Germplasm Enhancement/College of Horticulture/Gansu Key Laboratory of Aridland Crop Science, Gansu, Lanzhou 730070, China | | WANG Di | Gansu Key Laboratory of Crop Genetic and Germplasm Enhancement/College of Horticulture/Gansu Key Laboratory of Aridland Crop Science, Gansu, Lanzhou 730070, China
College of Agronomy, Gansu Agricultural University, Gansu, Lanzhou 730070, China |
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| Abstract: |
| This study investigated the morphological and physiological traits of eight transgenic potato (Solanum tuberosum L.) lines carrying AtNHX1 gene and one non-transgenic control “Gannongshu No.2” grown on normal soil and saline land at 0.56% salt and 0.29% salt concentration. The salt tolerance of transgenic potato was evaluated using a comprehensive evaluation method including subordinate function, correlation analysis and cluster analysis. The result showed that with the increase of salt concentration the plant height, leaf area expansion rate, yield per plant, chlorophyll content, activity of SOD and the rising range of proline content of transgenic lines were higher 1.17～3.96 times than that of the control. However, the increasing degree of MDA, relative conductivity of transgenic lines was significant lower than that of control, only 63.07%～78.4% and 82.7%～86.35% of the control. A transgenic potato line with highest salt tolerance and three transgenic lines with higher salt tolerance were screened out according to the comprehensive evaluation on the morphological, physiological and biochemical parameters. The transgenic lines with different degree of salt tolerance were as follows: 19>15>8>4>18>2>1>6>CK. The result concluded that the salt tolerance of transgenic potato grown in field can be improved by introducing AtNHX1 gene, and the comprehensive evaluation method can be used to screen out transgenic potato with high salt tolerance. |
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