| Effect of Sophora alopecuroides L. endophytic fungi on the growth of the host plant culture and synthesis of quinolizidine alkaloids |
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| DOI：10.7606/j.issn.1000-7601.2017.03.33 |
| Key Words: Sophora alopecuroides L. endophytic fungal fungal elicitor plant culture quinolizidine alkaloids |
| Author Name | Affiliation | | GAO Yuan | Agricultural College, Ningxia University, Yinchuan, Ningxia 750021, China
Huai'an Entry and Exit Inspection and Quarantine Bureau, Huai'an, Jiangsu 223001, China | | SUN Mu-di | Agricultural College, Ningxia University, Yinchuan, Ningxia 750021, China | | XU Quan-zhi | Agricultural College, Ningxia University, Yinchuan, Ningxia 750021, China | | LV Xi | Agricultural College, Ningxia University, Yinchuan, Ningxia 750021, China | | ZHANG Qing-chen | School of Pharmaceutical Sciences, Shandong University, Jinan, Shandong 250012, China | | Gu Pei-wen | Agricultural College, Ningxia University, Yinchuan, Ningxia 750021, China |
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| Abstract: |
| Eight strains of endophytic fungi were isolated and identified as the fungal elicitors from the healthy plant of S. alopecuroides L. and then their autoclaved mycelia and concentrated filtrates were made respectively to study the effect of different types, concentration and eliciting time of endophytic fungal elicitors on the growth of aseptic seedling and callus tissue of S. alopecuroides L., and the content of quinolizidine alkaloids. Among the 8 strains of endophytic fungal elicitors, the elicitation effect of the concentrated filtrate was stronger than those of the autoclaved mycelia. The autoclaved mycelia and concentrated filtrates of HMGKDF1 promoted the growth of callus, with the rate of growth being 1.82 and 1.42 times higher than that in CK, respectively. The concentrated filtrate of NDZKDF13 has a strong effect on the accumulation of quinolizidine alkaloids of S. alopecuroides L. The content of total alkaloids was 0.5483 mg·g-1, 23.8 times higher than that in CK. Within a certain range (0.01～1.0 mg·L-1), endophytic fungal elicitors promoted the synthetic accumulation of quinolizidine alkaloids of the host plant. After 12 days of induction by endophytic fungal elicitors, the content of quinolizidine alkaloids reached the highest amount, 2.65 times higher than that in CK. Addition of the autoclaved mycelia or concentrated filtrates from the endophytic fungi into aseptic seedlings and callus tissues of S. alopecuroides L. could be an effective way to increase the content of quinolizidine alkaloids and to improve the growth of the plant. |
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