| Study on method of actinomycetes isolation from soils of Taibai mountain and its ecological distribution |
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| DOI：10.7606/j.issn.1000-7601.2017.05.16 |
| Key Words: soil nutrient actinomycetes isolation method ecological distribution Taibai mountain |
| Author Name | Affiliation | | WANG He | College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi 712100, China | | PENG Chu | College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi 712100, China | | LAI Hang-xian | College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi 712100, China | | WEI Xiao-min | College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi 712100, China | | JIANG Ying-ying | College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi 712100, China |
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| Abstract: |
| The study explored the method of actinomycetes isolation from soils of Taibai mountain through five medias, including modification No.1 media、glycerin-asparagine media、R2A media、modification No.2 mediaand trehalose-proline media,and analyse its ecological distribution. The result showed that, soil nutrient of Taibai mountain on both north and south slope presented a relatively high concentration in mid-elevation forest type, the maximum concentration of organic matter and total nitrogen was 77.81 g·kg-1 and 5.35 g·kg-1, respectively, a relatively low concentration in the both sites of altitude, organic matter and total nitrogen concentration were lowest at 11.77 g·kg-1 and 0.85 g·kg-1, respectively, which means from low to high elevation in different forest types soil nutrient presented a trend of reducing then rising as a whole; Analysis of actinomycetes number and variety on different soils of plant types showed that modification glycerin-asparagine media was apparently higher than that from others, with the quantity of 258×104 cfu·g-1 soil, 35 species kinds, Modification No.1 media showed a better separation effect on the south slope, which mean more suitable for the separation of the south slope of different forest soil actinomyces. There are obvious advantages of R2A media while isolating mid-elevation forest type. Mid-elevation forest type, such as red birch、betula utilise in north slope, betula utilise in south slope, represented a significant difference than other types on the number of isolated actinomycetes. Soil available phosphorus and total nitrogen concentration had a greater influence on the number and variety isolated from soils, slope affected the correlation between isolation result and soil nutrients. |
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