| Effects of drought stress simulated by PEG6000 on physiological characteristics of different upland cotton |
|
View Fulltext View/Add Comment Download reader |
| |
| DOI：10.7606/j.issn.1000-7601.2017.05.33 |
| Key Words: cotton drought treatment seedling stage physiological indexes |
| Author Name | Affiliation | | HU Gen-hai | Henan Institute of Science and Technology, Collaborative Innovation Center of Modern Biological Breeding He'nan Province, Xinxiang, He'nan 453003, China | | DONG Na | Henan Institute of Science and Technology, Collaborative Innovation Center of Modern Biological Breeding He'nan Province, Xinxiang, He'nan 453003, China | | CHAO Mao-ni | Henan Institute of Science and Technology, Collaborative Innovation Center of Modern Biological Breeding He'nan Province, Xinxiang, He'nan 453003, China | | ZHANG Zhi-yong | Henan Institute of Science and Technology, Collaborative Innovation Center of Modern Biological Breeding He'nan Province, Xinxiang, He'nan 453003, China | | WANG Qing-lian | Henan Institute of Science and Technology, Collaborative Innovation Center of Modern Biological Breeding He'nan Province, Xinxiang, He'nan 453003, China |
|
| Hits: 1986 |
| Download times: 982 |
| Abstract: |
| In order to find useful indexes of the drought resistance cotton, treatment response of different cotton types seedlings was evaluated by using PEG6000 simulated drought. First, using matrix seedlings, and then the cotton seedlings were stressed by five different concentration of PEG. After the stress lasted for seven days, the content changes of chlorophyll, soluble sugar, soluble protein, proline and malondialdehyde of seedling leaves were measured, and the differences of peroxidase, superoxide dismutase and catalase activity were compared. The results showed that the cotton seedling presented different physiological characteristics under the stress of different concentration PEG stress, Chlorophyll content increased slightly under 5% PEG stress than the control, and chlorophyll content was lower than control in the 10% PEG stress; soluble sugar content showed higher levels under 10%～25% PEG stress; Soluble protein content increased more than control under 5% PEG stress; Proline content in leaves increased with the increase of stress concentration, But proline content of drought resistant and salt resistant varieties were higher than sensitive materials; malondialdehyde content in leaves had similar trends as proline, but in the range of 5%～15%, the drought resistant and salt resistant varieties were lower than the sensitive materials, under 20%, 25% PEG stress, the difference of three kinds of material was very small. Three kinds of antioxidant protection enzymes showed different change patterns of activity under different material treatments. The enzyme activity of peroxidase increased from 5% PEG stress, and the highest enzyme activity of peroxidase were displayed in 10%, 15% stress concentration. The enzyme activity of SOD in 5% PEG stress was higher than that of the control, and along with the increased PEG stress(10%,15%,20%), SOD enzyme activities were stable and keeping higher levels. The enzyme activity of CAT increased in low concentration PEG stress(5%,10%), and it decreased rapidly in more than 15% PEG stress. In conclusion, chlorophyll content, soluble protein, proline and malondialdehyde may be indexes of passive response of plants under drought stress. The soluble sugar is a kind of important osmotic regulation substance. In different levels of drought, different antioxidant protection enzymes play leading role, SOD enzyme may be a better drought resistance selection index. |
|
|
|