| QTL mapping of traits associated with drought resistance at bolting stage in Brassica napus |
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| DOI：10.7606/j.issn.1000-7601.2017.06.14 |
| Key Words: Brassica napus bolting stage genetic linkage map drought resistance QTL analysis |
| Author Name | Affiliation | | HUANG Qian | Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture/Oil Crops Research Institute, CAAS, Wuhan 430062, China
| | ZHAO Yong-guo | Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture/Oil Crops Research Institute, CAAS, Wuhan 430062, China
| | HUANG Xiang-wei | Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture/Oil Crops Research Institute, CAAS, Wuhan 430062, China
| | ZHU Zong-he | College of Agriculture, Anhui Agricultural University, Hefei 230036, China | | LIU Yun-qing | Agricultural Bureau of Xishui County, Xishui, Hubei 438200, China | | MA Hai-qing | Agricultural Bureau of Xishui County, Xishui, Hubei 438200, China | | CHENG Yong | Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture/Oil Crops Research Institute, CAAS, Wuhan 430062, China
| | ZOU Xi-ling | Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture/Oil Crops Research Institute, CAAS, Wuhan 430062, China
| | XU Jin-song | Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture/Oil Crops Research Institute, CAAS, Wuhan 430062, China
| | ZHANG Xue-kun | Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture/Oil Crops Research Institute, CAAS, Wuhan 430062, China
| | LU Guang-yuan | Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture/Oil Crops Research Institute, CAAS, Wuhan 430062, China
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| Abstract: |
| Bolting is one of the most sensitive stages to drought stress in Brassica napus. To identify quantitative trait loci(QTL) associated with drought resistance at bolting stage, we generated a F2∶3 populations with 183 lines by crossing drought-resistant line QY8-1 with the drought-sensitive one Huyou16. This population was subjected to two treatments, namely drought stress from bud emerging to bolting stages and well-water at the same stages. The results showed that shoot height, single plant fresh weight and leaf wilting index were all decreased by 52.6%～55.3% in average under drought stress compared to the control. Moreover, the three traits were highly positively correlated, indicating that they are suitable drought-resistant indicators. A linkage map was constructed with 335 SRAP markers, covering a total length of 1661.9 cM. By using composite interval mapping method, a total of 28 QTLs were detected across 14 linkage groups (LG), accounting for 1.1% to 36.6% of trait variation. Among these, 10 QTLs were identified for shoot height, and the one in LG15 (qSH-D-5) was a major QTL accounting for 36.6% of variation. There were three other QTLs（qSH-DRI-1、qSH-D-5 and qLWI-D-1） overlapping this interval, thus being an important genetic region for breeding selection via molecular markers. Meanwhile, there were also 4 minor QTLs overlapping at marker interval Em01/Me12-1—Em01Me09-1 in LG16, only accounting for 1.1%～8.1% of trait variation. |
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