| Effect of 5-azacytidine on photosynthesis of cucumber seedlings under low temperature and light intensity |
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| DOI：10.7606/j.issn.1000-7601.2017.06.18 |
| Key Words: cucumber seedling 5-azaC low temperature photosynthesis cold tolerance |
| Author Name | Affiliation | | XING Xiao-chen | Department of Horticulture, College of Agriculture, Shihezi University； Xinjiang Production and Construction Corps Key Laboratory of Special Fruits and Vegetables Cultivation Physiology and Germplasm Resources Utilization, Shihezi, Xinjiang 832003, China | | SUO Lin-ge | Department of Horticulture, College of Agriculture, Shihezi University； Xinjiang Production and Construction Corps Key Laboratory of Special Fruits and Vegetables Cultivation Physiology and Germplasm Resources Utilization, Shihezi, Xinjiang 832003, China | | XIAO Chun-yan | Department of Horticulture, College of Agriculture, Shihezi University； Xinjiang Production and Construction Corps Key Laboratory of Special Fruits and Vegetables Cultivation Physiology and Germplasm Resources Utilization, Shihezi, Xinjiang 832003, China | | LIU Miao-miao | Department of Horticulture, College of Agriculture, Shihezi University； Xinjiang Production and Construction Corps Key Laboratory of Special Fruits and Vegetables Cultivation Physiology and Germplasm Resources Utilization, Shihezi, Xinjiang 832003, China | | LIU Hui-ying | Department of Horticulture, College of Agriculture, Shihezi University； Xinjiang Production and Construction Corps Key Laboratory of Special Fruits and Vegetables Cultivation Physiology and Germplasm Resources Utilization, Shihezi, Xinjiang 832003, China | | CUI jin-xia | Department of Horticulture, College of Agriculture, Shihezi University； Xinjiang Production and Construction Corps Key Laboratory of Special Fruits and Vegetables Cultivation Physiology and Germplasm Resources Utilization, Shihezi, Xinjiang 832003, China |
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| Abstract: |
| The objective of this study was to explore the effect of reduction in methylation level of genomic DNA on photosynthesis of cucumber seedlings under low temperature. ‘Jinyan No.4’ cucumber seedlings was used as the experimental materials, and the effects of different concentrations of 5-azaC on the leaf gas exchange parameters, chlorophyll fluorescence quenching and cell membrane permeability of cucumber seedlings under low temperature (10℃/6℃, 300 μmol·m-2·s-1) were studied. The results showed that the net photosynthetic rate (Pn), stomatal conductance (Gs), transpiration rate (Tr), the maximum photochemical efficiency of PSⅡ(Fv/Fm), the actual photochemical efficiency (ΦPSⅡ), the photosynthetic electron transport efficiency (ETR) and the photochemical quenching coefficient (qP) of the cucumber seedlings leaf were increased, while leaf cell membrane permeability, intercellular CO2 concentration (Ci), the non-photochemical quenching coefficient (NPQ) and the leaf photochemical quenching parameters ［Y(NO)］ were reduced by 500 to 800 mol·L-1 of 5-azaC treatment under low temperature, thus improving the cold tolerance of cucumber seedlings. |
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