| Effect of salt stress and inhibitor on uptake and transportation of Na+ and K+ in the root of Ningxia Lycium barbarum L. |
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| DOI：10.7606/j.issn.1000-7601.2017.06.21 |
| Key Words: NaCl stress Lycium barbarum L. non-invasive micro-test technology Na+ K+ ion homeostasis inhibitors |
| Author Name | Affiliation | | ZHU Zhi-ming | Agricultural College of Ningxia University, Yinchuan, Ningxia 750021, China | | MAO Gui-lian | Life science College of Ningxia University, Yinchuan, Ningxia 750021, China | | XU Xing | Key Laboratory of Restoration and Reconstruction of the Ministry of Education, Northwest Degradation Ecosystem, Ningxia University, Yinchuan, Ningxia 750021, China | | WANG Sheng | Life science College of Ningxia University, Yinchuan, Ningxia 750021, China | | ZHENG Rui | Life science College of Ningxia University, Yinchuan, Ningxia 750021, China | | YANG Shu-juan | Life science College of Ningxia University, Yinchuan, Ningxia 750021, China |
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| Abstract: |
| We detected absorption and transportation of Na+, K+ in the root of Ningxia Lycium barbarum L. under NaCl stress by using non-invasive micro-test technology and atomic absorption spectrophotometry. The results showed that the root exhibited a higher capacity to induce the Na+ efflux compared with that of control. The higher the concentration of NaCl, the more Na+ accumulated in the root, but have no significant difference with CK. The K+ content increased first and then declined, and the Na+/K+ ratio showed the opposite trend. The longer duration of NaCl treatment, the more Na+ and K+ accumulated. At the mean time, the lower Na+ efflux and higher K+ efflux were also observed in the root compared with that of control. These results suggested that initial salt stress enhanced the salt-elicited Na+ efflux but inhibited K+ efflux to maintain the lower Na+/K+ ratio and cellular Na+/K+ homeostasis. The NaCl-induced Na+ efflux and H+ influx were inhibited by amiloride (a Na+/H+ antiporter inhibitor) or sodium orthovanadate (a plasma membrane H+-ATPase inhibitor), indicating that Na+ extrusion in the root of Ningxia Lycium barbarum L. arises from the active Na+/H+ antiporter across the plasma membrane. |
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