| Effects of Streptomyces griseus on soil enzyme activity, nutrients and microorganism of the seedlings of Platycodon grandiflorum rhizosphere under drought stress |
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| DOI：10.7606/j.issn.1000-7601.2017.06.26 |
| Key Words: the seedlings of Platycodon grandiflorum Streptomyces griseus drought stress soil enzyme activity soil nutrient microbial community |
| Author Name | Affiliation | | LI Dui-shu | College of Biopharmaceutical Engineering and Food Science, Shangluo University, Shangluo, Shaanxi 726000, China | | HE Nian-wu | College of Biopharmaceutical Engineering and Food Science, Shangluo University, Shangluo, Shaanxi 726000, China | | JI Yu-liang | College of Biopharmaceutical Engineering and Food Science, Shangluo University, Shangluo, Shaanxi 726000, China |
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| Abstract: |
| Platycodon grandiflorum of rhizosphere soil under drought stress was treated with different concentrations of Streptomyces griseus fermentation medium and different concentrations of Streptomyces griseus fermentation broth (original solution, and original solution diluted 10, 50, 100 times(The number of bacteria was 1×108 cfu·mL-1) and 150 times), mixed with 0～20 cm of the soils in 200 mL pots respectively, sterile water was as control (CK). Soil water holding capacity was controlled around 45%. When the Platycodon grandiflorum growth reached four leaves, the rhizosphere soil (0～20 cm) was taken at 7, 14, 21, 28, and 35 days. The changes of rhizosphere soil invertase, urease, catalase, cellulase and protease activities were measured with colorimetry and potassium permanganate titration, and the number of microbes were determined by dilution method of plate counting. The results showed that the activity of invertase, urease, catalase, cellulase and protease in the rhizosphere soil of Platycodon grandiflorum increased with the increase of the dilution of the fermentation broth of Streptomyces griseus. When the fermented broth was diluted to 100 times, peak of invertase, urease, catalase, cellulase and protease activities reached 68%, 12.63%, 11.53%, 128.24%, 43.51% respectively higher than that of CK. Platycodon grandiflorum rhizosphere soil treated by Streptomyces griseus fermentation broth diluted 100 times, significantly improved soil organic matter, total nitrogen, available P, available K contents by 280.13%, 99.15%, 60%, 60.12% respectively higher than CK at 35 d. The bacteria in soil treated by Streptomyces griseus fermentation broth continuously increased, in by 355.43% than CK at 35 d. Actinomyces and fungi in the soil increased first and then decreased, but were all higher than CK. Correlation analysis of soil enzyme activities, soil nutrient factors and soil microorganisms showed that urease and organic matter, total N, available P, available K, bacteria were significantly positive correlated (P＜0.01). Cellulase and organic matter, total N, available P, available K, bacteria, actinomycetes were extremely significant or significantly positive correlated. Protease and effective phosphorus, rapidly-available potassium, bacteria, actinomyces, fungi were extremely significant or significant positive correlated . Invertase, peroxidase (pod) and organic matter, total N, available P, available K, bacteria were not significant and negative correlated, but invertase, peroxidase and actinomyces, fungi had a positive correlation but not significant. |
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