| Impact of irrigation with saline water on soil enzyme activity and soil enzyme kinetics |
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| DOI：10.7606/j.issn.1000-7601.2018.01.15 |
| Key Words: saline water irrigation soil enzyme activities soil enzyme kinetics inhibition mechanism |
| Author Name | Affiliation | | ZHAI Hong-mei | Key Laboratory of Agricultural Water Resources, Chinese Academy of Sciences
Hebei Key Laboratory of Agricultural Water-Saving
Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang, Hebei 050022, China
College of Chemistry, Shijiazhuang University, Shijiazhuang, Hebei 050035, China | | CAO Cai-yun | Dry farming Research Institute of Hebei Academy of Agricultural and Forestry Sciences, Hengshui, Hebei 053000, China | | LIU Meng-yu | Key Laboratory of Agricultural Water Resources, Chinese Academy of Sciences
Hebei Key Laboratory of Agricultural Water-Saving
Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang, Hebei 050022, China |
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| Abstract: |
| In order to explore the reason of the decline of soil quality caused by salt water irrigation, the field experiment with five levels of irrigation water salinity (1.25、 2.50、 5.00、 7.50 dS·m-1 and 10.00 dS·m-1) was conducted. The impact of saline water on soil enzyme activity and soil enzyme kinetics was studied. The results showed that irrigation with saline water reduced the activities of soil urease, β-glucosidase, and alkaline phosphatase. Compared with the control, they were decreased by 45.5%, 50.8% and 24.9% in 0～20 cm, 37.44%、32.1% and 21.8% in 20～40 cm, respectively, by 10 dS·m-1 saline water. Irrigation with 10 dS·m-1 saline water affected kinetic characters of soil enzymes. The maximum reaction rates (Vmax) of urease, β-glucosidase and alkaline phosphatase were decreased by 47.77%, 44.62% and 15.01%, respectively. The Michaelis constant (Km) of urease and alkaline phosphatase decreased by 57.12% and 14.64%, while the Km of β-glucosidase did not vary significantly. Vmax/Km of β-glucosidase was decreased by 39.63%. However, Vmax/Km of alkaline phosphatase and urease did not changed significantly. |
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