| Effect of ethyl methane sulphonate on agronomic traits and quality of winter rapeseed in North China |
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| DOI：10.7606/j.issn.1000-7601.2018.01.32 |
| Key Words: Brassica rapa L winter rapeseed EMS mutagenesis agronomic traits quality |
| Author Name | Affiliation | | YANG Jian-sheng | Gansu Key Laboratory of Crop Improvement and Germplasm Enhancement/Key Laboratory of Arid Land Crop Science in Gansu Province, Lanzhou, Gansu 730070, China | | SUN Wan-cang | Gansu Key Laboratory of Crop Improvement and Germplasm Enhancement/Key Laboratory of Arid Land Crop Science in Gansu Province, Lanzhou, Gansu 730070, China
Rapeseed Engineering Research Center of Gansu Province, College of Agronomy, Gansu Agricultral University, Lanzhou, Gansu 730070, China | | LIU Zi-gang | Gansu Key Laboratory of Crop Improvement and Germplasm Enhancement/Key Laboratory of Arid Land Crop Science in Gansu Province, Lanzhou, Gansu 730070, China
Rapeseed Engineering Research Center of Gansu Province, College of Agronomy, Gansu Agricultral University, Lanzhou, Gansu 730070, China | | LIU Hai-qing | Gansu Key Laboratory of Crop Improvement and Germplasm Enhancement/Key Laboratory of Arid Land Crop Science in Gansu Province, Lanzhou, Gansu 730070, China | | YANG Gang | Gansu Key Laboratory of Crop Improvement and Germplasm Enhancement/Key Laboratory of Arid Land Crop Science in Gansu Province, Lanzhou, Gansu 730070, China | | ZHANG Shu-juan | Gansu Key Laboratory of Crop Improvement and Germplasm Enhancement/Key Laboratory of Arid Land Crop Science in Gansu Province, Lanzhou, Gansu 730070, China | | ZHANG Jing | Gansu Key Laboratory of Crop Improvement and Germplasm Enhancement/Key Laboratory of Arid Land Crop Science in Gansu Province, Lanzhou, Gansu 730070, China | | FANG Yan | Gansu Key Laboratory of Crop Improvement and Germplasm Enhancement/Key Laboratory of Arid Land Crop Science in Gansu Province, Lanzhou, Gansu 730070, China
Rapeseed Engineering Research Center of Gansu Province, College of Agronomy, Gansu Agricultral University, Lanzhou, Gansu 730070, China | | WU Jun-yan | Gansu Key Laboratory of Crop Improvement and Germplasm Enhancement/Key Laboratory of Arid Land Crop Science in Gansu Province, Lanzhou, Gansu 730070, China
Rapeseed Engineering Research Center of Gansu Province, College of Agronomy, Gansu Agricultral University, Lanzhou, Gansu 730070, China | | LI Xue-cai | Gansu Key Laboratory of Crop Improvement and Germplasm Enhancement/Key Laboratory of Arid Land Crop Science in Gansu Province, Lanzhou, Gansu 730070, China
Rapeseed Engineering Research Center of Gansu Province, College of Agronomy, Gansu Agricultral University, Lanzhou, Gansu 730070, China |
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| Abstract: |
| In order to explore ethyl methane sulphonate (EMS) mutagenesis on agronomic traits and qualities in winter rapeseed (Brassica rapa L.), and to enrich germplasm of winter rapeseed in north China, Longyou 7 was treated with ethyl methane sulfonate (EMS) at different concentrations (0, 0.3%, 0.6%, 0.9%, 1.2%). Morphological characteristics in M1 and influence of agronomic traits and qualities of M2 were measured. The result showed that EMS mutagenesis on winter rapeseed produced abundant variation, which included leaves appear chimerism bleaching, petals albino, the female and stamens deformity. The height, length of main inflorescence, pods of main inflorescence, the pods per plant, pod length, seeds per pod, 1000-seed weight, all of these decreased significantly except the number of branches. In addition large variation produced in fatty acid content. Screening resulted in three plants with higher oleic acid content and low erucic acid content in M2, and one mutant with low glucosinolates content (21.97 μmol·g-1). Proper EMS concentration is suggested as being 0.3%～0.6%, and the treating time is 6 h. |
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