| Effect of drought resistance of Bromes irradiated by 60Co-γ-ray in the seed germination stage |
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| DOI：10.7606/j.issn.1000-7601.2018.02.18 |
| Key Words: bromes PEG stress 60Co-γ- ray drought resistance |
| Author Name | Affiliation | | LI Bo | College of Agriculture, Forestry and Life Sciences, Qiqihar University, Qiqihar, Heilongjiang 161006, China | | LI Xiang-li | College of Agriculture, Forestry and Life Sciences, Qiqihar University, Qiqihar, Heilongjiang 161006, China |
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| Abstract: |
| In order to explain the effect of water tolerance of 60Co-γ radiation on the seed germination of Bromes, polyethylene glycol (PEG-6000) was used to simulate water stress, The seeds of Bromes are used as materials,using germination box filter paper germination. the effects of different 60Co-γ radiation on the seeding growth、cell membrane permeability、osmotic adjustment and the activity of protective enzymes were studied. The results showed that：10% PEG was the best stress concentration in the seed germination period of Bromes. Low dose radiation to improve the root of the seed water absorption capacity, and promote the water content of seedling, there by promotion seedling growth, high-dose radiation is inhibited. But the relative conductivity content was positively correlated with the radiation dose, the radiation dose gradually increased, the destruction of the cell membrane is enhanced. The contents of proline, soluble sugar and soluble protein were negatively correlated with the radiation dose. Low dose radiation could promote the osmotic adjustment of substance and maintain normal physiological metabolism. With the increase of radiation dose, the protective enzyme activities of Bromes were all increased first and then decreased, which indicated that low dose radiation could promote the free radical scavenging ability of Bromes, and the high dose had inhibitory effect. Low dose radiation can promote promotes its ability of scavenging radicals of Bromes, while the high dose has inhibitory effect. Thus, the low dose of 60Co-radiation (50～100 Gy) to promote the growth of Bromes and protected the cell membrane by the decrease of relative conductivity. At the same time, there is no increase in osmotic regulation and antioxidant enzyme activity to adapt to the water stress environment Inhibition, the degree of damage to a minimum. |
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