| Monosaccharide composition of polysaccharides in Ziziphus jujuba Mill cv. lingwuchangzao fruit |
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| DOI：10.7606/j.issn.1000-7601.2018.02.22 |
| Key Words: Ziziphus jujuba Mill cv.lingwuchangzao fruit polysaccharides monosaccharide composition |
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| Abstract: |
| The fruit of Ziziphus jujuba Mill cv. lingwuchangzao in different developmental periods was used as material to study the change of polysaccharides content and monosaccharide composition, using methods of hot water extraction, spectrophotometry and gas chromatography-mass spectrometry. The main research results are as follows: the crude polysaccharides in Ziziphus jujuba Mill cv. lingwu-changzao fruits of different developmental periods were extracted by hot water extraction, the crude polysaccharides obtaining ratio is different, the crude polysaccharides obtaining ratio in the early bulking period is 0.778%, the rapid enlargement period 0.865%, the coloring period 0.983%, and the fruit maturation period 1.795%. The crude polysaccharide at different developmental periods was fractionated to four fractions (Ju-0、Ju-1、Ju-2、Ju-3) by DEAE-52 cellulose ion-exchange columns, and was purified to be refined polysaccharides by HW-55S molecular size exclusion chromatography. Finally, the refined polysaccharides in the different developmental periods of Ziziphus jujuba Mill cv. lingwuchangzao were analysed by the GC-MS, the monosaccharide composition and content show that the contents of arabinose, galactose and rhamnose are higher, followed by glucose, mannose and xylose, but the monosaccharide composition and contents varied with polysaccharides component. In addition, the refined polysaccharide in the different developmental periods of Ziziphus jujuba Mill cv. lingwuchangzao also contained ribose, fuctose, glucuronic acid and galacturonic acid. In summary, the crude polysaccharide obtaining ratio and contents present ascendant trend with the development of the fruits, and reach the peak 1.795% at the fruit maturation period. The refined polysaccharide contents of Ziziphus jujuba Mill cv. lingwuchangzao fruits at different developmental periods varied according to component. |
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