| Identification and evaluation of salt-tolerant sorghum germplasm resources |
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| DOI：10.7606/j.issn.1000-7601.2018.03.11 |
| Key Words: grain sorghum salt tolerance identification and evaluation cluster analysis principal component analysis |
| Author Name | Affiliation | | FAN Na | Sorghum Research Institute of Shaanxi Academy of Agricultural Sciences, Shaanxi, Yuci 030600
Sorghum genetic and germplasm innovation in Shaanxi Province key laboratory, Shaanxi, Yuci 030600 | | BAI Wen-bin | Sorghum Research Institute of Shaanxi Academy of Agricultural Sciences, Shaanxi, Yuci 030600
Sorghum genetic and germplasm innovation in Shaanxi Province key laboratory, Shaanxi, Yuci 030600 | | PENG Zhi-dong | Sorghum Research Institute of Shaanxi Academy of Agricultural Sciences, Shaanxi, Yuci 030600
Sorghum genetic and germplasm innovation in Shaanxi Province key laboratory, Shaanxi, Yuci 030600 | | ZHAO Jian-wu | Sorghum Research Institute of Shaanxi Academy of Agricultural Sciences, Shaanxi, Yuci 030600
Sorghum genetic and germplasm innovation in Shaanxi Province key laboratory, Shaanxi, Yuci 030600 |
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| Abstract: |
| 50 sorghum parents material were used to study the seed germination, seedling growth and salt tolerance of different sorghum materials, using Cluster Analysis and Principal Component Analysis. By correlation analysis, principal component analysis and cluster analysis, This work provides the theoretical basis for salt-tolerant sorghum selection and salt tolerance research, and to provide material for salt-tolerant gene mining of sorghum varieties. The experimental results showed that, under the stress of 150mmol·L-1 NaCl, the growth index of sorghum parental materials is lower than the control, Different varieties had different reduction levels. The relative germination rate ranged from 0.27 to 93.4%, the relative germination potential ranged from 15.15% to 97.4%, the root length range was from 0.3 to 4.5 cm, the leaf length range was from 0.4 to 2.8 cm, and the salt injury coefficient range was 0.026 to 0.794.. The rate of germination rate is positively correlated with germination rate and germination potential, and the correlation coefficient is 0.98***. According to the principal component analysis, the first principal component is the germination growth factor; the second principal component is the growth factor, and the third principal component is the salt tolerance factor. The cluster analysis divided 30 sorghum strains into five categories: high-salt-tolerant, salt tolerant, moderate-salt-tolerant, salt-sensitive and high-salt-sensitive. Among which 3560R, 10337B and iron 10612 belong to the salt-sensitive category, and 09305R and 67B are high-salt products. In this study, it also showed that sorghum germination rate, germination potential, shoot length, root length, seedling height, growth rate, fusarium wilt index, germination rate salt damage coefficient and germination potential salt damage coefficient can be used as important indexes for sorghum salt tolerance. |
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