| Mitigation effect analysis of different exogenous regulatory substances on maize under low-temperature stress |
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| DOI：10.7606/j.issn.1000-7601.2018.03.28 |
| Key Words: maize low-temperature stress exogenous Pro Glycine betaine(GB) mitigation index comprehensive evaluation |
| Author Name | Affiliation | | ZHAO Xiao-qiang | Gansu Provincial Key Laboratory of Aridland Crop Science/College of Agronomy, Gansu Agricultural University, Lanzhou, Gansu 730070, China | | PENG Yun-ling | Gansu Provincial Key Laboratory of Aridland Crop Science/College of Agronomy, Gansu Agricultural University, Lanzhou, Gansu 730070, China | | FANG Peng | Gansu Provincial Key Laboratory of Aridland Crop Science/College of Agronomy, Gansu Agricultural University, Lanzhou, Gansu 730070, China | | WU Bo-yang | Gansu Provincial Key Laboratory of Aridland Crop Science/College of Agronomy, Gansu Agricultural University, Lanzhou, Gansu 730070, China | | YAN Hui-ping | Gansu Provincial Key Laboratory of Aridland Crop Science/College of Agronomy, Gansu Agricultural University, Lanzhou, Gansu 730070, China |
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| Abstract: |
| Proline (Pro) and Glycine betaine (GB) are important osmoregulation substances that are associated with cold resistance in plants. To investigate the effect of exogenous Pro and GB on maize germination and seedling traits under low-temperature stress, and to comprehensively evaluate the cold mitigation effect of Pro and GB. Three maize chilling sensitive lines were used with five different concentrations of Pro or GB’s soak or culture treatment under 10℃ stress at germination or seedling stage. Double distilled water soak and culture were used as positive and negative control check (CK(+) and CK(-)) under 25℃ and 10℃ at two stages. It showed that: (1) The germination potential (GP), germination rate (GR), plumule length (PL), radical length (RL), shoot fresh weight (SFW), root fresh weight (RFW) of maize seed significantly decreased, and relative conductivity (RC), malondialdehyde content (MDA), proline content (Pro), soluble sugar content (SS), SOD, POD and CAT activity of seedling significantly increased under low-temperature stress. (2) RC and MDA significantly decreased, and other five traits of seedling and six traits of seed germination significantly increased after adding exogenous Pro and GB under low-temperature stress. (3) The six germination traits and seven physiological and biochemical traits showed significant difference in inbred lines, in the concentrations of Pro and GB, and in the interaction of inbred lines and concentration of Pro and GB. (4) Cold ease index (EI) was used as comprehensive evaluation index, and membership function method was used to comprehensive evaluate the exogenous Pro and GB’s mitigation effect to colde stress. 600, 800μmol·L-1 of Pro and 20μmol·L-1 of GB had the best cold mitigation effect of 0.882 and 0.647, and 1000μmol·L-1 of Pro and 50μmol·L-1 of GB had the worst cold mitigation effect of 0.513 and 0.022 at germination. 400μmol·L-1 of Pro and 10μmol·L-1 of GB had the best cold mitigation effect of 0.577 and 0.500, and 1000μmol·L-1 of Pro and 40μmol·L-1 of GB had the worst cold mitigation effect of 0.246 or 0.289 at seedling stage. The cold mitigation effect of Pro was 1.858 and 1.064 times of GB at germination and seedling stage, respectively. |
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