| Effects of NaN3 and ultraviolet combined mutation on the growth of alfalfa callus under salt and drought stress |
|
View Fulltext View/Add Comment Download reader |
| |
| DOI：10.7606/j.issn.1000-7601.2018.04.27 |
| Key Words: Alfalfa callus NaN3 and ultraviolet combined mutation salt stress drought stress |
| Author Name | Affiliation | | LI Bo | College of Agriculture，Forestry and Life Sciences，Qiqihar University, Heilongjiang Provincial Key Laboratory of Resistance Gene Engineering and Protection of Biodiversity in Cold Areas, Heilongjiang, Qiqihar 161006，China | | LIN Hao | College of Agriculture，Forestry and Life Sciences，Qiqihar University, Heilongjiang Provincial Key Laboratory of Resistance Gene Engineering and Protection of Biodiversity in Cold Areas, Heilongjiang, Qiqihar 161006，China | | WU Ting-ting | College of Agriculture，Forestry and Life Sciences，Qiqihar University, Heilongjiang Provincial Key Laboratory of Resistance Gene Engineering and Protection of Biodiversity in Cold Areas, Heilongjiang, Qiqihar 161006，China | | CHEN Xue-mei | College of Agriculture，Forestry and Life Sciences，Qiqihar University, Heilongjiang Provincial Key Laboratory of Resistance Gene Engineering and Protection of Biodiversity in Cold Areas, Heilongjiang, Qiqihar 161006，China |
|
| Hits: 1177 |
| Download times: 794 |
| Abstract: |
| Alfalfa callus of Tumu No.2 was used as experimental material, callus was mutated with different concentrations of NaN3 and different dose of UV, and treated with NaCl and PEG stress at different concentrations. The growth status of the callus was observed. The semi lethal(LC50) and lethal(LC100) concentration (dose) of each treatment was determined according to the survival rate of callus. The content of proline, soluble sugar, MDA and the activity of CAT in callus were assayed. The results showed that the growth of alfalfa callus was affected by mutation and stress.The survival rate of callus had declined with the increase of NaN3 concentration and UV irradiation dose.The semi lethal and lethal concentrations of NaN3 was 4.0 and 5.5×10-3 mol·L-1 respectively. At the distance of 20 cm, the semi lethal and lethal doses of UV induced mutation was 6 and 8 min. The survival rate of callus declined with the concentration of NaCl and PEG increasing. The lethal and lethal concentrations of NaCl was 1.5% and 2% respectively. The lethal and lethal concentrations of PEG was 25% and 30% respectively. Compared with different mutation and stress treatments, the survival rate of callus was 16% treated by NaN3 and UV combined mutagenesis and PEG and NaCl double stress treatment. The content of proline, soluble sugar, CAT enzyme increased and MDA content decreased in the callus under NaN3 and UV combined mutagenesis. Screening of alfalfa callus by mutagenesis and stress could improve the salt and drought resistance. It provides the basis for using somatic cells to expand the resources of anti-stress variation in alfalfa. |
|
|
|