Effects of light intensity on germination, growth, chlorophyll content and photosystem II of leaves in Ryegrass(Lolium Perenne L) |
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DOI:10.7606/j.issn.1000-7601.2018.04.30 |
Key Words: ryegrass(Lolium Perenne L) light intensity germination and growth chlorophyii content PSII |
Author Name | Affiliation | Zhang Hui | Key Laboratory of Disaster Survey and Mechanism Simulation of Shaanxi Province/Baoji University of Arts and Sciences, Baoji, Shaanxi 721013, China | Wang He | Key Laboratory of Disaster Survey and Mechanism Simulation of Shaanxi Province/Baoji University of Arts and Sciences, Baoji, Shaanxi 721013, China | Zhang Bei-bei | Key Laboratory of Disaster Survey and Mechanism Simulation of Shaanxi Province/Baoji University of Arts and Sciences, Baoji, Shaanxi 721013, China | Wu Yue-xuan | Key Laboratory of Disaster Survey and Mechanism Simulation of Shaanxi Province/Baoji University of Arts and Sciences, Baoji, Shaanxi 721013, China | Wang Miao-miao | Key Laboratory of Disaster Survey and Mechanism Simulation of Shaanxi Province/Baoji University of Arts and Sciences, Baoji, Shaanxi 721013, China |
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Abstract: |
Using common ryegrass(Lolium Perenne L) as a test material, the effects of six different light intensities on germination, growth, chlorophyll content and photosystem II of leaves in ryegrass were studied. The results showed that: (1)When the light intensity was 300 μmol·m-2·s-1, the ryegrass was the best for germination and growth. The germination rate, germination potential, germination index and vigor index were 93%, 23.67%, 57.28 and 211.88, respectively. The root length and shoot length were 3.04 cm and 4.01 cm, respectively; the chlorophyll content of leaves under this light intensity was significantly higher than that of other light intensity treatments (P<0.05), and the chlorophyll a, b and total chlorophyll were 0.71, 0.27 mg·g-1 and 0.98 mg·g-1; (2) With the increase of light intensity, the maximum fluorescence (Fm), the maximum photochemical efficiency of PSII (Fv/Fm) and PIabs showed a trend of increasing first and then decreasing, while the values of Fm, Fv/Fm and PIabs reached the maximum under light intensity 300 mol·m-2·s-1; OJIP was greatly affected by light intensity, with significant difference at point I, and the OJIP curve was normalized with little difference.; (4) OJIP curve parameters showed that there was no significant difference in light intensity of the six donor groups on the PSII, with the increase of light intensity, per reaction center absorption (ABS/RC), capture (TRo/RC) and transfer (ETo/RC) also increases, but the heat dissipation (DIo/RC) per reaction center decreases, and the electron transfer of PSII receptor side is blocked at light intensity 500 μmol·m-2·s-1. |
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