16S rDNA of rhizobium isolated from faba bean of different ecotopes in Qinghai Province
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DOI:10.7606/j.issn.1000-7601.2018.04.37
Key Words: faba bean, rhizobium, 16S rDNA, phylogeny
Author NameAffiliation
ZHANG Xiao-juan (College of Eco-Environmental Engineering, The Open Project of State Key Laboratory of Plateau Ecology and AgricultureQinghai University, Xining, Qinghai 810016 
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Abstract:
      It has been proved to be benefitial for utilization of faba bean-rhizobium system. To clarify genetic diversity of faba bean rhizobia in Qinghai province and discover new rhizobiums, we isolated rhizobiums from Qingcan14 and used 16S rDNA to analyze the six isolated strains. Blast results showed a high similarity between the six strains and reported sequences. Among them, CD4 showed 98% similarity with KF008225.1, and the remaining strins showed a similarity ranging from 95% to 96%. The six strains belonged to four genus, Arthrobacter (CD1 and CD2), Mycobacterium (CD3), Rhizobium (CD4 and CD5), Mesorhizobium (CD6), which were diverse between ecotopes. The cluster results showed that six strains belonged to five branches, indicating the high genetic diversity of rhizobiums in Qinghai province.