| Transgenic CarNAC1 gene improves the drought resistance of cotton |
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| DOI：10.7606/j.issn.1000-7601.2018.04.39 |
| Key Words: CarNAC1 transgenic cotton drought resistance cotton yield fiber quality |
| Author Name | Affiliation | | WANG Shu-jun | College of Agronomy/Key Laboratory of Agricultural Biotechnology,Xinjiang Agricultural University, Urumqi 830052 | | QU Yan-ying | College of Agronomy/Key Laboratory of Agricultural Biotechnology,Xinjiang Agricultural University, Urumqi 830052 | | NI Zhi-yong | College of Agronomy/Key Laboratory of Agricultural Biotechnology,Xinjiang Agricultural University, Urumqi 830052 | | WANG Li-ping | College of Agronomy/Key Laboratory of Agricultural Biotechnology,Xinjiang Agricultural University, Urumqi 830052 | | GAO Wen-wei | College of Agronomy/Key Laboratory of Agricultural Biotechnology,Xinjiang Agricultural University, Urumqi 830052 | | CHEN Quan-jia | College of Agronomy/Key Laboratory of Agricultural Biotechnology,Xinjiang Agricultural University, Urumqi 830052 |
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| Abstract: |
| In this paper, 15 stable gebetically transgenic cotton plants with CarNAC1 gene and the receptor materials K62 were used for filed and laboratory tests, cotton photosynthesis index, agronomic traits, yield traits and fiber quality and physiological indicators were compared by weighted membership function method, principal component analysis and cluster analysis. The results showed that after drought stress, the stomatal conductance decreased and net photosynthetic rate of the transgenic cotton lines was significantly higher than that of the receptor material K62. the average boll weight of the transgenic cotton lines was higher than that of the receptor material 0.363~0.657 g; In addition the content of MDA was lower than the receptor material of K62 transgenic cotton lines, proline, soluble sugar content and catalase, peroxidase and superoxide dismutase activity in transgenic cotton lines were higher than that of the receptor material. Based on the results of principal components analysis of agronomic traits and yield traits indicators, the 9 indexes of 16 lines were integrated into 2 independent comprehensive indexes, which combined with D value and cluster analysis results can still maintain relatively high yield after drought stress. The lines: ZK62-10, ZK62-6, ZK62-1. Baesed on the results of principal components analysis of fiber quality index, the 7 indicators of 16 lines were integrated into 3 independent comprehensive indexes, combined with D value and cluster analysis results were used ro screen out the relatively high fiber quality levels after drought stress. the lines: ZK62-6, ZK62-12, ZK62-15. After drought stress, the yield and quality of transgenic cotton lines carrying CarNAC1 gene were better than that of receptor K62. CarNAC1 transgenic cotton lines may improve the drought resistance of cotton by reducing stomatal conductance and enhancing the osmotic conditions. The best cotton line with comprehensive drought resistance ability was screened out: ZK62-6. |
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