Effects of salt stress on endogenous hormone contents in sunflower seedlings |
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DOI:10.7606/j.issn.1000-7601.2018.06.14 |
Key Words: sunflower salt stress endogenous hormones ELISA |
Author Name | Affiliation | LI Hai-yang | School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang, Sichuan 621000, China Beijing Agricultural Intelligent Equipment Technology Research Center, Beijing 100097, China | LI Ai-xue | Beijing Agricultural Intelligent Equipment Technology Research Center, Beijing 100097, China | WANG Cheng | Beijing Agricultural Intelligent Equipment Technology Research Center, Beijing 100097, China | WANG Xiao-dong | Beijing Agricultural Intelligent Equipment Technology Research Center, Beijing 100097, China | HOU Pei-chen | Beijing Agricultural Intelligent Equipment Technology Research Center, Beijing 100097, China | LI Wen-jiao | School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang, Sichuan 621000, China | CHEN Ke | School of Life Science and Engineering, Southwest University of Science and Technology, Mianyang, Sichuan 621000, China |
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Abstract: |
The hydroponic cultured sunflower seedlings after 20d was treated in 0, 50, and 100 mmol·L-1 salt solution for 0, 10, 20, and 30 h as salt stress treatments. The concentrations of endogenous hormones in roots, stems, and leaves of sunflower seedlings were determined by enzyme-linked immunosorbent assay (ELISA). The results indicated that with increasing salt concentrations and stress time, the four hormones in root, stem, and leaves of the seedlings showed inconsistent trends. The content of IAA, ABA, and ZR increased first and then decreased. The greater the salt concentration, the greater the amplitude of the increase or decrease in the hormone contents and the faster the change rate in the hormones content. GA did not change significant and fluctuated within the ±2.0 ng·g-1 range during salt stress treatment. The ratio of IAA/ABA, GA/ABA, and (ZR+IAA+GA) /ABA in roots, stems, and leaves of sunflowers showed an overall downward trend, indicating salt stress inhibited sunflower growth. It is shown that under salt stress, the changes in concentrations of these hormones in leaves and stems of sunflower seedlings were greater than that in roots. |
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