NaN3诱变和盐碱胁迫对苜蓿愈伤组织生长和生理特性的影响

李波; 邬婷婷

Effects of NaN₃ mutagenesis and saline-alkali stress on growth and physiological characteristics of alfalfa callus

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DOI：10.7606/j.issn.1000-7601.2019.02.19

Key Words: alfalfa callus NaN₃ mutagenesis saline alkali stress survival rate biomass physiology characteristics

Author Name	Affiliation
LI Bo	College of Agriculture，Forestry and Life Sciences，Heilongjiang Provincial Key Laboratory of Resistance Gene Engineering and Protection of Biodiversity in Cold Areas，Qiqihar University, Qiqihar,Heilongjiang 161006, China
WU Ting-ting	College of Agriculture，Forestry and Life Sciences，Heilongjiang Provincial Key Laboratory of Resistance Gene Engineering and Protection of Biodiversity in Cold Areas，Qiqihar University, Qiqihar,Heilongjiang 161006, China

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Abstract:

In order to decipher the effect of NaN₃ mutagenesis and saline-alkali stress on the growth and physiological characteristics of alfalfa callus, we conducted an experiment that the growth and physiological indexes of alfalfa callus, “Zhongmu No.1”were treated by NaN₃ and saline-alkali (NaCl, NaHCO₃, and Na₂CO₃) stresses. The results showed that increasing NaN₃ and salt concentration decreased the survival rate of callus. When the concentration of NaN₃ was at 3 mmol·L^-1, 8 d treatment decreased the survival rate of callus to 54.55%. This treatment combination is NaN₃ mutagenic semi-lethal conditions. The survival rate of callus was lower than 5% at 200 mmol·L^-1of NaN₃ after 12 d treatment that is the lethal concentration of each salt and stress time. In addition to the low concentration of NaCl stress group, the biomass of the callus decreased with the increase of NaN₃ and salt stress concentration, NaN₃ treatment decreased the biomass in a range of 60.85% to 81.88%, the NaCl stress group decreased that by 13.90%~65.63%, the NaHCO₃ stress group decreased that by 21.71%~67.37%, and the Na₂CO₃ stress group decreased that by 61.54%~73.20%. The effect of salt and alkali on the biomass of alfalfa callus ranked in this order: NaCl> NaHCO₃> Na₂CO₃. The content of soluble protein, soluble sugar and proline in the callus increased significantly, and the content of MDA and electrical conductivity decreased by NaN₃ mutation and salt stress. The soluble protein content in treatments of NaN₃, NaN₃+NaCl, NaN₃+ Na₂CO₃ and NaN₃+ NaHCO₃ increased by 47.07%, 53.57%, 22.31%, and 47.69%, respectively. The soluble sugar content increased by 24.79%, 162.86%, 58.36% and 109.54%, respectively.The proline content increased by 47.78%, 162.86%, 80.00%, and 107.25%, respectively. The MDA content decreased by 2.35%, 11.17%, 3.64%, 5.54%, respectively. The relative conductivity decreased by 15.13%, 17.96%, 16.79% and 17.28% in all treatments, respectively. The NaN₃ induced the accumulation of osmotic substances in the callus and decreased the permeability of membrane.