| Screening of chemical-resistant Streptomyces ZZ-9 after microwave mutagenesis |
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| DOI：10.7606/j.issn.1000-7601.2019.02.37 |
| Key Words: screening of microwave mutagenesis resistance strain streptomyces ZZ-9 synergistic effect |
| Author Name | Affiliation | | CHEN Da-wei | College of Plant Protection, Gansu Agricultural University and Bio-control Engineering Laboratory of Crop Diseases and Pests, Lanzhou, Gansu 730070, China | | XUE Ying-yu | College of Plant Protection, Gansu Agricultural University and Bio-control Engineering Laboratory of Crop Diseases and Pests, Lanzhou, Gansu 730070, China | | SHEN Zhi-yan | College of Plant Protection, Gansu Agricultural University and Bio-control Engineering Laboratory of Crop Diseases and Pests, Lanzhou, Gansu 730070, China | | MAO Wei-xing | College of Plant Protection, Gansu Agricultural University and Bio-control Engineering Laboratory of Crop Diseases and Pests, Lanzhou, Gansu 730070, China | | ZHANG Shu-wu | College of Plant Protection, Gansu Agricultural University and Bio-control Engineering Laboratory of Crop Diseases and Pests, Lanzhou, Gansu 730070, China | | XU Bing-liang | College of Plant Protection, Gansu Agricultural University and Bio-control Engineering Laboratory of Crop Diseases and Pests, Lanzhou, Gansu 730070, China |
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| Abstract: |
| In order to obtain thiophanate-methyl resistant mutant strains of Streptomyces ZZ-9, the original strain Streptomyces ZZ-9 with low resistance to thiophanate-methyl was mutated using microwave for 0 to 75 s, which could grow well under the lethal concentration of thiophanate-methyl. After genetic stability test of resistance and antagonism to Cytospora sp., ideal resistant strains were screened out. The combination of resistant strains and thiophanate-methyl was used to treat Valsa canker on apple trees. The results showed that with a mutation time of 15 to 60 s, 4 stains with chemicals-resistance capability named ZZ-9A, ZZ-9B, ZZ-9C and ZZ-9D were obtained. After measuring the inhibition rate and culturing several generations, a strain named ZZ-9B with relatively stable hereditary and better inhibition rate was screened out. The synergistic effect of the strain ZZ-9B and 70 μg·mL-1 of thiophanate-methyl showed a 89.42% effectiveness on the apple branches, which was significantly higher than sole use of stain ZZ-9B or thiophanate-methyl. The optimal ratio of the strain ZZ-9B to thiophanate-methyl was 8∶2. It is concluded that small amount of thiophanate-methyl with the strain ZZ-9B can significantly improve the biocontrol efficiency of Valsa canker on apple tree. |
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