| Screening and identification of Medicago Sativa Linn growth promoting rhizobacteria under saline-alkali stress |
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| DOI：10.7606/j.issn.1000-7601.2019.02.38 |
| Key Words: saline-alkali tolerance PGPR screening and identification of strains saline-alkali soil Medicago Sativa Linn |
| Author Name | Affiliation | | XIANG Jun-liang | College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing， Heilongjiang 163319, China | | TANG Cheng-rui | Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, Liaoning 110016, China | | WANG Jia-qi | College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing， Heilongjiang 163319, China | | LIU Quan | College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing， Heilongjiang 163319, China | | ZHANG Xing-mei | College of Agriculture, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China | | YIN Kui-de | College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing， Heilongjiang 163319, China |
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| Abstract: |
| In order to ameliorate and utilize saline-alkali soil, the saline-alkali soil was used to separate the saline-alkali-tolerant bacteria under pH 8.5, 50 mmol·L-1 Na2CO3 and 100 mmol·L-1 NaHCO3. The growth-promoting function of the strain was tested by CAS, ADF, PKO and other functional medium. The 16S rDNA sequence analysis was used to identify the strains. The effect of the bacteria on the alfalfa seed germination rate, germination potential and seedling growth were investigated under pH 8.5 and 25 mmol·L-1 Na2CO3. The results showed that strain S11 could produce IAA and ACC deaminase, and it was identified as Bacillus horikoshii. Under the saline-alkali condition and after inoculation of strain S11, alfalfa seed germination rate and germination potential were increased by 15% and 10%, respectively. The root length, plant height, fresh biomass, and chlorophyll content were increased by 34.78%、12.5%、27.3% and 43.30%, respectively. This proved that strain S11 could relieve saline-alkali stress and promote the growth of alfalfa. |
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