| Evaluation of salt tolerance of 26 Eruca sativa Mill. |
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| DOI：10.7606/j.issn.1000-7601.2019.03.02 |
| Key Words: Eruca sativa salt tolerance physiological characteristics subordinate function value method |
| Author Name | Affiliation | | FAN Hui-ling | College of Agriculture and Biotechnology Hexi University, Zhangye, Gansu 73400, China | | LIU Qin | Zhangye Academy of Agricultural Sciences, Zhangye, Gansu 734000, China | | BAI Sheng-wen | College of Agriculture and Biotechnology Hexi University, Zhangye, Gansu 73400, China | | LUO Tian | College of Agriculture and Biotechnology Hexi University, Zhangye, Gansu 73400, China | | ZHU Xue-feng | College of Agriculture and Biotechnology Hexi University, Zhangye, Gansu 73400, China | | Miu Chun-qing | Zhangye Academy of Agricultural Sciences, Zhangye, Gansu 734000, China |
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| Abstract: |
| To assess the salt tolerance of 26 Eruca sativa Mill., 6 concentration of NaCl solution were used，including 0, 0.2%, 0.4%, 0.6%, 0.8% and 1.0%, to treat Eruca sativa at germination stage, and the germination rate, radicles length were measured. 0.6% and 0.8% NaCl solution were selected to treat the seedling of Eruca sativa, the physiological indexes of seedling growth and salt tolerance were detemined, and all resources were sorted out using the method of membership function. Results showed that: (1) Tests on seed germination indicated that stress of 0.6% NaCl had an inhabitation of 50% nearly, salt stress of 0.8% NaCl basically caused growth stagnation， therefore, these two concentrations were the thresholds of salt stress inhibiting the growth of Eruca sativa; (2) Survival seedlings, shoot height, root length, fresh weight of shoot and root were all decreased with the increase of salt concentration, the mean were 57.62%～34.45%, 0.99～0.77 cm, 0.69～0.44 cm, 0.0034～0.0023 g, 0.0244～0.0176 g, respectively; (3) Based on the membership function on the means of parameters at seedling stages for each growth level, 26 materials were classified into three types: high salt tolerance, including 08 wuyun 3-1, etc (0.6133≤the verage of membership function values≤0.7261); salt tolerance, including Lintao yunjie, etc (0.4274≤the verage of membership function values≤0.5911); low salt tolerance, including 13 yunjie 76-4,etc (0.0280≤the verage of membership function values≤0.3894); (4) The contents of free proline and soluble sugar in high salt tolerant meaterials were significantly higher than those in low salt tolerant materials. In conclusion, significant difference in salt tolerance of 26 Eruca sativa were revealed, the salt tolerant materials screened by method of membership function of fuzzy mathematics could be further used in salt-tolerant breeding and gene mining. |
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