| Physiological defense response of promoting synthesis and accumulation of host alkaloid by endophytic fungal elicitors in Sophora alopecuroides L. |
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| DOI：10.7606/j.issn.1000-7601.2019.03.07 |
| Key Words: Sophora alopecuroides L. endogenous fungal elicitors oxymatrine defense enzyme activity tissue culture seedling |
| Author Name | Affiliation | | SUN Mu-di | Agricultural College, Ningxia University, Yinchuan, Ningxia 750021, China | | HU Li-jie | Agricultural College, Ningxia University, Yinchuan, Ningxia 750021, China | | LI Wen-xue | Agricultural College, Ningxia University, Yinchuan, Ningxia 750021, China | | YAN Si-yuan | Agricultural College, Ningxia University, Yinchuan, Ningxia 750021, China | | ZHANG Zhong | Wine College, Ningxia University, Yinchuan, Ningxia 750021, China | | GU Pei-wen | Agricultural College, Ningxia University, Yinchuan, Ningxia 750021, China |
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| Abstract: |
| Four species of endophytic fungi were isolated from Sophora alopecuroides L., and their concentrated filtrates were prepared as the fungal elicitors. The effects of different endophytic fungal elicitors at different induction time on the content of oxymatrine (OMA) and the activities of phenylalanine(PAL), peroxidase (POD), ascorbate peroxidase (APX) and catalase (CAT) in the tissue culture seedlings of S. alopecuroides L. were investigated. The results showed that culture broth concentrates from 4 species of endophytic fungi of S. alopecuroides L. obviously improved the content of OMA in tissue culture seedlings of S. alopecuroides L. Among them, culture broth concentrates of the endophytic fungi XKGKDF40 was most effective with 0~15 d of induction, OMA content in tissue culture seedlings of S. alopecuroides L. continuously increased, compared with the control, and reached the maximum of 3.616 mg·g-1 on the 15th day, which was 2.09 times that of the control group with the same induction time. Culture broth concentrates from 4 species of endophytic fungi of S. alopecuroides L. induced increase in activity of important PAL enzyme in alkaloid synthesis of tissue culture seedlings of S. alopecuroides L., and promoted the activities of antioxidant enzymes, such as POD, APX and CAT. The endophytic fungi XKGKDF40 was the most efficient elicitor on PAL activation, the enzyme activity reached a peak of 61.92 U·g-1 at 6th day after induction, which was 1.42 times that of the control group at the same induction time; Compared with other elicitors, the elicitor XKZKDF27 could induce POD activity significantly, after the induction in 12th day, POD activity reached the maximum of 62.30 U·mg-1, which is 2.70 times that of the control group at the same induction time; The elicitor XKZKDF11 also greatly improved the activities of APX and CAT in tissue culture seedlings of S. alopecuroides L., the enzyme activities reached the highest activity at the 12th day of induction (0.5538 U·mg-1 and 19.82 U·mg-1), which were 4.97 times and 3.00 times that of the control group at the same period respectively. The results indicated that the addition of endophytic fungal elicitors from S. alopecuroides L. not only activated the defensive response of the host tissue culture seedlings, but also promoted the synthesis of OMA of S. alopecuroides L.. |
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