| Effects of different doses of 60Co-γ ray on seed germination and seedling growth characteristics of Echinochloa crusgalli |
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| DOI：10.7606/j.issn.1000-7601.2019.03.08 |
| Key Words: Echinochloa crusgalli seed 60Co-γ radiation seed germination seedling growth correlation analysis principal component analysis |
| Author Name | Affiliation | | JIA Xiu-feng | College of Education and Media，Qiqihar University，Qiqihar，Heilongjiang 161006，China | | LI Bo | College of Agriculture, Forestry and Life Sciences, Heilongjiang Provincial Key Laboratory of Resistance Gene Engineering and Protection of Biodiversity in Cold Areas, Qiqihar University,Qiqihar, Heilongjiang 161006, China | | LI Hong | Institute of Animal Husbandry and Veterinary of Heilongjiang Academy of Agricultural Sciences, Qiqihar, Heilongjiang 161005, China | | WU Ting-ting | College of Agriculture, Forestry and Life Sciences, Heilongjiang Provincial Key Laboratory of Resistance Gene Engineering and Protection of Biodiversity in Cold Areas, Qiqihar University,Qiqihar, Heilongjiang 161006, China | | MA He | College of Agriculture, Forestry and Life Sciences, Heilongjiang Provincial Key Laboratory of Resistance Gene Engineering and Protection of Biodiversity in Cold Areas, Qiqihar University,Qiqihar, Heilongjiang 161006, China |
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| Abstract: |
| Echinochloa crusgalli(zhaomu No.1) seed were used as experimental materials, and seeds were treated with different doses （50、100、150、200、250、300、350、400、450 Gy） of 60Co-γ ray.The germination rate, germination potential, germination index, vigor index,main root length, bud length, total length of bud seedling, fresh weight, dry weight,relative water content of bud,seed germination rate and seedling survival rate were measured. The correlation and principal component analyses on various indicators during seed germination were conducted. The results showed that with the increase inradiation dosage,the germination rate with middle and high radiation doses decreased by 3.34%~25.00%, the germination potential increased by 6.84%~41.09%, the germination index increased by 1.84%~33.50%, the vigor index decreased by 8.87%~62.86%,the bud length decreased by 9.97%~56.78%, the main root length decreased by 18.48%~70.52%, the total length of bud seedling decreased by 11.25%~62.20%,the fresh weight (except 150 Gy) decreased by 0.32%~34.50%, the dry weight decreased by 2.91%~11.58%, the relative water content increased by 29.37%~57.54%, the 50~350 Gy seed germination rate increased by 3.12%~18.75%, and the seedling survival rate of 50~300 Gy decreased by 5.13%~94.87%.After different doses of 60Co-γ radiation, germination rate, vigor index, bud length, main root length, total length of bud seedlings and fresh weight were significantly positively correlated with seedling survival rate. According to the eigenvector value of more than 0.30 as the standard, the main indicators of seed germination and seedling growth of Echinochloa crusgalli were bud length, total length of bud seedling, main root length, survival rate, vigor index, germination rate, relative water content and germination rate. |
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