The effect of nitrogen stress on carbon invertase enzyme activities in tartary buckwheat seedling stage
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DOI:10.7606/j.issn.1000-7601.2019.04.18
Key Words: tartary buckwheat  low nitrogen  seedling stage  soil  β-glucosidase  sucrase  cellulase
Author NameAffiliation
CHEN Wei College of Geographical Sciences, Shanxi Normal University, Linfen, Shanxi 041000, China 
YANG Yang College of Geographical Sciences, Shanxi Normal University, Linfen, Shanxi 041000, China 
CUI Ya-ru College of Geographical Sciences, Shanxi Normal University, Linfen, Shanxi 041000, China 
SUN Cong-jian College of Geographical Sciences, Shanxi Normal University, Linfen, Shanxi 041000, China 
ZHANG Yong-qing College of Geographical Sciences, Shanxi Normal University, Linfen, Shanxi 041000, China
College of Life Sciences, Shanxi Normal University, Linfen, Shanxi 041000, China 
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Abstract:
      In order to investigate the carbon invertase enzymes activities under different nitrogen (N) tolerant tartary buckwheat to low N stress in seedling stage, using Diqing tartary buckwheat (low N tolerance, DQ) and Heifeng No. 1 (low N sensitive, HF) as materials. The study was carried out in a pot experiment to evaluate the different N treatments of no N (CK), low N (N1) and normal N (N2) as the treatments. The physicochemical properties and three carbon invertase activities of tartary buckwheat seedlings were measured. The results showed that: (1) DQ had species advantages under low N stress. Plant height, stem diameter, leaf area, soil organic carbon and available N were significantly higher than that of HF, while soil moisture content and pH were lower than that of HF. (2) Both N fertilizer treatments and cultivars had significant effects on the activities of carbon related enzymes involved in the transformation in tartary buckwheat growing soil. The activities of the monosaccharide hydrolase (β-glucosidase) and disaccharidease hydrolase (sucrose) in DQ were significantly higher than that of HF under low N treatment, the monosaccharide enzyme of DQ was 58.3% higher than that of HF, and the disaccharide enzyme was 3.3 times higher than that of HF. Under normal N treatment, both enzyme activities with DQ were 19.7% and 42.8% lower than that with HF, respectively. Under different N treatments, DQ secreted more polysaccharide enzymes (cellulase) and were 66%, 40% and 22% higher than HF under 3 N treatments, respectively. In summary, the varieties of tartary buckwheat with strong ability to tolerate low N can adapt to the low N growth environment by regulating the soil enzyme activities, and may change their preference for carbon sources in this way.
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