Isolation and identification of P-dissolving fungi strain and its effects on phosphate-solubilizing and plant growth promotion
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DOI:10.7606/j.issn.1000-7601.2019.04.34
Key Words: phosphate-solubilizing fungi  dissolving characteristics  promoting effect  tomato
Author NameAffiliation
XUE Ying-yu College of Plant Protection, Gansu Agricultural University/Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province, Lanzhou, Gansu 730070, China 
YE Wei College of Plant Protection, Gansu Agricultural University/Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province, Lanzhou, Gansu 730070, China 
YANG Shu College of Plant Protection, Gansu Agricultural University/Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province, Lanzhou, Gansu 730070, China 
LI Pei College of Plant Protection, Gansu Agricultural University/Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province, Lanzhou, Gansu 730070, China 
XU Bing-liang College of Plant Protection, Gansu Agricultural University/Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province, Lanzhou, Gansu 730070, China 
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Abstract:
      In order to improve phosphorus of soil and provide P-solubilizing microbial resource for bio-fertilizer production,a fungi P-1407 with strong phosphate-solubilizing ability was isolated from rhizosphere soil of Nitraria tangutorum in Minqin desert-oasis ecotone by using flat plate separation method. The dissolving characteristics, acid production, and resistance to salt-alkaline of strain P-1407 were also studied.The results showed that the strain P-1407 had high-efficiency of phosphate-solubilizing capability for Ca3(PO4)2 during a 10d shaking incubation. The maximum activity of phosphate-dissolving was reached at 557 μg·mL-1. Also, Its P solubilizing capacity was negatively related to soil pH.It was revealed that the strain P-1407 mainly secreted gluconic acid, oxalic acid, and formic acid using HPLC analysis.The strain P-1407 was stable under alkaline and high salt conditions. According to its cultural characteristics, micromorphological characteristics, and ITS rDNA sequence, the strain P-1407 was identified as Penicillium oxalicum.The pot culture experiment results showed that the germination rate of tomato seed treated with P-1407 increased by 2.53% compared with that of the control, and the plant height, root length, fresh weight of aboveground parts, dry weight of aboveground parts, fresh weight of underground parts and dry weight of underground parts were increased by 109.27%, 109.27%, 82.95%, 121.69%, 82.95%, and 121.69%, respectively.Therefore,the strain P-1407 is a promising microbial resource for development of high efficiency biological phosphorus fertilizer of soil in the future.
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