Identification of salinity tolerant mutant strain of Trichoderma sp. by ultraviolet mutagenesis
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DOI:10.7606/j.issn.1000-7601.2019.04.35
Key Words: Trichoderma sp.  ultraviolet mutagenesis  NaCl stress  growth  salt tolerance
Author NameAffiliation
ZHANG Shu-wu College of Plant Protection, Gansu Agricultural University / Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province, Lanzhou, Gansu 730070, China 
XU Bing-liang College of Plant Protection, Gansu Agricultural University / Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province, Lanzhou, Gansu 730070, China 
LIU Jia College of Plant Protection, Gansu Agricultural University / Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province, Lanzhou, Gansu 730070, China 
SHI Cheng-cai College of Plant Protection, Gansu Agricultural University / Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province, Lanzhou, Gansu 730070, China 
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Abstract:
      In order to obtain high efficient salt tolerant mutant strains of Trichoderma sp., we used the original strain of Trichoderma atroviride T-YM, which was isolated from the salinity soil to obtain the mutant strains by ultraviolet (UV) mutagenesis, and screened the ultraviolet mutagenesis parameters for the mutant strains, as well as determined the ability of salt tolerant parameters of colony diameters, dry weight and quantity of spore production for the mutant strains under different concentrations of NaCl solution. The results showed that the colony diameters and quantity of spore production by mutant strains were significantly higher after UV mutagenesis for 3 min. than that of original strain, and the colony diameters and quantity of spore production were increased by 17.69% and 28.82%, respectively, compared with the control (original strain). The dry weight of mycelia was significantly higher at 0.5 and 5 min, and was increased by 42.22% and 46.67%, respectively. Moreover, the colony diameters, dry weight, and quantity of spore production of mutant strains were higher than that of the original strain under NaCl stress. The average quantity of spore production was increased by 51.18% at 3 min, and the dry weight of mycelia was increased by 23.65% under different concentrations of NaCl solution. At the same time, the salt-tolerance of mutant strain was relatively stable after incubation for ten generations under NaCl stress (10 mg·mL-1) after UV mutagenesis for 0.5 min. Therefore, UV mutagenesis can be considered as an efficient method for screening and breeding the salt tolerant strains of Trichoderma sp..
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