| Cloning and expression analysis of three glutathione S-transferase GST genes from sunflower (Helianthus annuus) |
|
View Fulltext View/Add Comment Download reader |
| |
| DOI：10.7606/j.issn.1000-7601.2019.05.14 |
| Key Words: Helianthus annuus glutathione S-transferase GST gene gene clone relative gene expression |
| Author Name | Affiliation | | LING Yun-he | College of Agriculture,Northwest Agriculture and Forestry University,Yangling,Shanxi 71210, China | | LI Jing | College of Agriculture,Northwest Agriculture and Forestry University,Yangling,Shanxi 71210, China | | JING Bing | College of Agriculture,Northwest Agriculture and Forestry University,Yangling,Shanxi 71210, China | | LI Chun-lian | College of Agriculture,Northwest Agriculture and Forestry University,Yangling,Shanxi 71210, China | | XIAO En-shi | College of Agriculture,Northwest Agriculture and Forestry University,Yangling,Shanxi 71210, China | | WANG Zhong-hua | College of Agriculture,Northwest Agriculture and Forestry University,Yangling,Shanxi 71210, China |
|
| Hits: 1253 |
| Download times: 478 |
| Abstract: |
| The plant glutathione-S-transferase (GST, EC2.5.1.18) is a group of enzymes with multiple biological functions encoded by complex gene families that has great responses to various abiotic stresses. In this study, three GST genes responding to salt stress were obtained from sunflower transcriptome sequencing results and a phylogenetic tree were constructed for analysis. These three genes belong to Tau GST and were named as HaGSTU26 (HanXRQChr04g0127901), HaGSTU8 (HanXRQChr06g0177581) and HaGSTU27(HanXRQChr10g0316331). Next, we cloned these three genes from sunflower and performed expression analysis under different tissues and different stress conditions. Sequence analysis showed that DNA sequence of HaGSTU26 gene was 1674bp and the CDS was 666 bp, which encoded 221 amino acids and consisted of 2 exons and 1 intron. The DNA sequence of HaGSTU8 gene was 2271 bp and the CDS was 654 bp, which encoded 217 amino acids and consisted of 2 exons and 1 intron. The DNA sequence of HaGSTU27 gene and CDS were both 663bp, encoding 220 amino acids and consisting of one exon. Real-time fluorescence quantitative PCR analysis showed that the expression levels of HaGSTU26, HaGSTU8 and HaGSTU27 sunflower were different in different tissues (root, young leaf, mature leaf, stem, young stem and temporal leaf), the HaGSTU26 gene and HaGSTU27 gene had the highest expression in roots, while HaGSTU8 gene had the highest expression in the temporal leaf. However, all three genes have the lowest expression in mature stems. Under different stress conditions, the expression levels of these three genes in sunflower seedlings were determined. The results showed that under salt and ABA stress, the amount of gene expression increased first and then decreased with increasing treatment time. Under salt stress, the relative expression of HaGSTU26 gene was at peak after 12h, and the expression of HaGSTU8 and HaGSTU27 were at the highest after 3 h. After ABA stress, the expression levels of HaGSTU26 and HaGSTU27 were the highest after 12 h, and the relative expression of HaGSTU8 was the highest after 24 h. Under cold stress, HaGSTU26 and HaGSTU27 genes were up-regulated, and they had the highest relative expression after 3 h and 24 h, respectively. The expression of HaGSTU8 gene was down-regulated, and its relative expression decreased gradually with the prolongation of treatment time. Under heat stress, the relative expression of three genes increased with the prolongation of stress time, and the highest expression was obtained after 24 h. The above results indicate that these three genes respond to different abiotic stresses (salt, ABA, cold and heat stress). |
|
|
|