Effects of fertilization on metabolic function of microorganisms and growth of maize at seedling stage in reclaimed soil
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DOI:10.7606/j.issn.1000-7601.2019.05.26
Key Words: coal gangue reclamation soil  fertilization  soil microbial functional diversity  maize growth
Author NameAffiliation
ZHANG Bian-hua Xinzhou Teachers CollegeXinzhou, Shanxi 034000, China
Institute of Agricultural Resources and Environment, Shanxi Academy of Agricultural Sciences, Taiyuan, Shanxi 030031, China 
JIN Dong-sheng Institute of Agricultural Resources and Environment, Shanxi Academy of Agricultural Sciences, Taiyuan, Shanxi 030031, China 
ZHANG Qiang Institute of Agricultural Resources and Environment, Shanxi Academy of Agricultural Sciences, Taiyuan, Shanxi 030031, China 
GAO Chun-hua Institute of Agricultural Resources and Environment, Shanxi Academy of Agricultural Sciences, Taiyuan, Shanxi 030031, China 
LI Jian-hua Institute of Agricultural Resources and Environment, Shanxi Academy of Agricultural Sciences, Taiyuan, Shanxi 030031, China 
LU Jin-jing Institute of Agricultural Resources and Environment, Shanxi Academy of Agricultural Sciences, Taiyuan, Shanxi 030031, China 
JI Shen-yu Institute of Agricultural Resources and Environment, Shanxi Academy of Agricultural Sciences, Taiyuan, Shanxi 030031, China 
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Abstract:
      In this paper, Biolog-ECO technology, WinRHIZO scanner and image analysis technology were used to study metabolic function of soil microbial community and root morphology of maize at seedling stage in the reclamation of coal gangue under CK (0 fertilizer), compound fertilizer F (600 kg·hm-2), organic fertilizer O (7 500 kg·hm-2), organic-inorganic compound fertilizer OF (300 kg·hm-2+3 750 kg·hm-2) treatments. The results showed that the Shannon-wiener index and the Simpson index were the highest at 3.22 and 0.96, respectively, and the Eveness index of 0.21 was the lowest under OF treatment while CK was opposite, Shannon-wiener index and the Simpson index were the lowest at the values of 3.07 and 0.95, respectively, the Eveness index peaked at 0.23. The key carbon source that affected the metabolic function diversity of microbial communities mainly included six kinds of amino acids (L-arginine, L-asparagine, L-phenylalanine, L-serine, L-threonine and glycyl-L-glutamic acid), four kinds of esters (D-galactosate gamma-lactone, methyl pyruvate, Twain 40, Twain 80), one amine (humus), five kinds of acids (D-galactosonic acid, 2-hydroxybenzoic acid, 4-hydroxybenzoic acid, r-hydroxybutyric acid, D-malic acid), three kinds of sugars (Methyl-glucoside, glucose-1-phosphate, a-cyclic dextrin), three kinds of alcohols (I-alginol, D, L-a-glycerol, D-mannitol); OF treatment had great differences from CK on the aspects of maize root morphology such as root length, root volume, root fractal dimension and its biological traits such as biomass, stem thickness and height. It was indicated that OF treatment had the best effects on corn growth, and improved soil quality in the coal gangue reclamation.