| Identification of salt\|tolerant germplasm and analysis of its physiological response during barley germination |
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| DOI：10.7606/j.issn.1000-7601.2019.06.03 |
| Key Words: barley germination stage salt tolerance physiological response identification evaluation |
| Author Name | Affiliation | | SUN Jian | Gansu Provincial Key Lab of Aridland Crop Science/Gansu Provincial Key Lab of Crop Improvement & Germplasm Enhancement, Lanzhou, Gansu 730070, China
College of Agronomy, Gansu Agricultural University, Lanzhou, Gansu 730070, China | | XU Xiaoyun | Gansu Provincial Key Lab of Aridland Crop Science/Gansu Provincial Key Lab of Crop Improvement & Germplasm Enhancement, Lanzhou, Gansu 730070, China
College of Agronomy, Gansu Agricultural University, Lanzhou, Gansu 730070, China | | MA Zengke | Gansu Provincial Key Lab of Aridland Crop Science/Gansu Provincial Key Lab of Crop Improvement & Germplasm Enhancement, Lanzhou, Gansu 730070, China
College of Agronomy, Gansu Agricultural University, Lanzhou, Gansu 730070, China | | HE Jianjun | Gansu Provincial Key Lab of Aridland Crop Science/Gansu Provincial Key Lab of Crop Improvement & Germplasm Enhancement, Lanzhou, Gansu 730070, China
College of Agronomy, Gansu Agricultural University, Lanzhou, Gansu 730070, China | | WANG Juncheng | Gansu Provincial Key Lab of Aridland Crop Science/Gansu Provincial Key Lab of Crop Improvement & Germplasm Enhancement, Lanzhou, Gansu 730070, China
College of Agronomy, Gansu Agricultural University, Lanzhou, Gansu 730070, China | | YAO Lirong | Gansu Provincial Key Lab of Aridland Crop Science/Gansu Provincial Key Lab of Crop Improvement & Germplasm Enhancement, Lanzhou, Gansu 730070, China
College of Agronomy, Gansu Agricultural University, Lanzhou, Gansu 730070, China | | LI Baochun | Gansu Provincial Key Lab of Aridland Crop Science/Gansu Provincial Key Lab of Crop Improvement & Germplasm Enhancement, Lanzhou, Gansu 730070, China
College of Life Sciences and Technology, Gansu Agricultural University, Lanzhou, Gansu 730070, China | | MA Xiaole | Gansu Provincial Key Lab of Aridland Crop Science/Gansu Provincial Key Lab of Crop Improvement & Germplasm Enhancement, Lanzhou, Gansu 730070, China
College of Agronomy, Gansu Agricultural University, Lanzhou, Gansu 730070, China | | SI Erjing | Gansu Provincial Key Lab of Aridland Crop Science/Gansu Provincial Key Lab of Crop Improvement & Germplasm Enhancement, Lanzhou, Gansu 730070, China
College of Agronomy, Gansu Agricultural University, Lanzhou, Gansu 730070, China | | YANG Ke | Gansu Provincial Key Lab of Aridland Crop Science/Gansu Provincial Key Lab of Crop Improvement & Germplasm Enhancement, Lanzhou, Gansu 730070, China
College of Agronomy, Gansu Agricultural University, Lanzhou, Gansu 730070, China | | WANG Huajun | Gansu Provincial Key Lab of Aridland Crop Science/Gansu Provincial Key Lab of Crop Improvement & Germplasm Enhancement, Lanzhou, Gansu 730070, China
College of Agronomy, Gansu Agricultural University, Lanzhou, Gansu 730070, China | | MENG Yaxiong | Gansu Provincial Key Lab of Aridland Crop Science/Gansu Provincial Key Lab of Crop Improvement & Germplasm Enhancement, Lanzhou, Gansu 730070, China
College of Agronomy, Gansu Agricultural University, Lanzhou, Gansu 730070, China |
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| Abstract: |
| In order to understand the salt tolerance and physiological response characteristics of barley germination, 126 barley subjects were treated with 200 mmol·L-1 of NaCl to evaluate its effect on seed germination and seedlings growth in hydroponic experiments on extreme salt tolerant materials and salt sensitive materials. The results showed that the measured values of barley germination during salt stress decreased and the coefficient of variation increased compared with the control. The coefficient of variation of germination potential, germination rate, plant height, root length and root number were 73.81%, 58.00%, 47.22%, 39.39% and 31.79%, respectively, indicating that the salt stress treatment of different materials was quite different; and there was no correlation between plant height and germination rate or root length. However, among other indicators, there was significant or extremely significant positive correlation. Clustering analysis was used to classify the materials into four salt tolerance categories, among which 33 varieties including ZDM655 were highly salt tolerant, 28 varieties including MAVRIIP were moderately salt tolerant; 16 varieties including GN241 were moderately salt sensitive, and 49 varieties including Putian 17 were salt sensitive. Under salt stress hydroponic conditions, barley mainly adapted to salt stress by adjusting root surface area and root volume phenotypic characteristics. Highly salt\|tolerant materials, ZDM655, had Na+ content in the shoots and roots of 3.63 and 2.55 times of the control, respectively, while the Na+ content in the shoots and roots of the salt\|sensitive material ZDM222 was 8.95 and 2.92 times of the control, respectively. Meanwhile, K+ content in aboveground parts of ZDM655 was higher than that of the control and K+ content in underground parts decreased, while K+ content in ZDM222 shoot significantly decreased while that in underground portion was not significantly different compared with the control, indicating that highly salt tolerant material had phenotypic characteristics and physiological response function of better adaptation to salt stress. The salt tolerance during seed germination is the key to growth in saline soil. Germination potential, germination rate, plant height, root length, and root number can be used as reasonable indicators for salt tolerance evaluation of barley germination, and excellent for salted land excavation. |
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