| Northeast Institute of Geography and Agroecology, CAS, Harbin, Heilongjiang 150081, China |
|
View Fulltext View/Add Comment Download reader |
| |
| DOI：10.7606/j.issn.1000-7601.2019.06.25 |
| Key Words: microbial community functional diversity continuous cropping AWCD carbon source metabolism fertilization |
| Author Name | Affiliation | | YAN Jun | Northeast Institute of Geography and Agroecology, CAS, Harbin, Heilongjiang 150081, China | | HAN Xiaozeng | Northeast Institute of Geography and Agroecology, CAS, Harbin, Heilongjiang 150081, China | | CHEN Xu | Northeast Institute of Geography and Agroecology, CAS, Harbin, Heilongjiang 150081, China | | ZOU Wenxiu | Northeast Institute of Geography and Agroecology, CAS, Harbin, Heilongjiang 150081, China | | LU Xinchun | Northeast Institute of Geography and Agroecology, CAS, Harbin, Heilongjiang 150081, China | | HAO Xiangxiang | Northeast Institute of Geography and Agroecology, CAS, Harbin, Heilongjiang 150081, China |
|
| Hits: 1203 |
| Download times: 480 |
| Abstract: |
| In order to determine the effect of continuous cropping on the functional diversity of soil microbial community under the condition of long\|term fertilization and non\|fertilization. A typical black soil with the treatments of continuous cropping of wheat, maize, and soybean under long\|term fertilization and non\|fertilization from long\|term position experimental station were selected as the research object in this study. Dilution plate method and Biolog Eco microplate method were used to determine the counts of soil bacteria, fungi, and actinomycetes and carbon metabolism, which benefit for built the optimal management. The results showed that the number of bacteria, fungi, and actinomycetes in continuous cropping of soybean, wheat, and maize was higher under fertilization treatments than that under non\|fertilization treatments, and the highest counts of bacteria and fungi were observed under the soybean continuous cropping. The counts of bacteria under the treatments with wheat and maize continuous cropping decreased by 24.8% and 31.0%, the number of fungi decreased by 64.0% and 51.2% respectively than the treatments with soybean continuous cropping under the condition of no\|fertilization. While the counts of bacteria under wheat and maize continuous cropping decreased by 29.0% and 45.5%, the counts of fungi decreased by 26.7% and 31.5%, respectively, under the fertilization. The results of Biolog showed that the average well color development (AWCD) of wheat continuous cropping was higher than that of maize and soybean continuous cropping, and that of soybean continuous cropping was higher than that of wheat and maize continuous cropping. Carbohydrate was the most utilized carbon source in soybean, maize, and wheat continuous cropping under the no\|fertilization, while carbohydrate and polymer were the most utilized in different continuous cropping systems after fertilization. In the soybean and wheat continuous cropping, α-D-lactose was the most efficient carbon source with no fertilization, while D, l-α-glycerin was the most efficient carbon source with the fertilization. D-galacturonic acid was the highest carbon source of soil microbial utilization under maize continuous cropping whether fertilizing or not. Glucose-1-phosphate and γ-hydroxybutyric acid were the key carbon sources for the specific utilization of the microbial community in the black soil. The results of principal component analysis indicated that the effect of fertilization on soil microbial carbon metabolism was greater than on crops. |
|
|
|