| Effect of chicken manure and its co-application with urea on aroma substances, aroma-related enzyme activities and gene expression of oriental melon |
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| DOI：10.7606/j.issn.1000-7601.2019.06.27 |
| Key Words: oriental sweet melon chicken manure enzyme activities aroma substance gene expression |
| Author Name | Affiliation | | JIN Yazhong | College of Horticulture and Landscape Architecture, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China | | CHEN Yewen | College of Horticulture and Landscape Architecture, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China | | LU Miao | College of Horticulture and Landscape Architecture, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China | | ZHANG Sen | College of Horticulture and Landscape Architecture, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China | | LAN Hui | College of Horticulture and Landscape Architecture, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China | | HE Shuping | College of Horticulture and Landscape Architecture, Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang 163319, China | | Qi Hongyan | College of Horticulture, Shenyang Agricultural University, Shenyang, Liaoning 110866, China |
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| Abstract: |
| An experiment was conducted to study the effects of chicken manure and its co-application with urea on aroma components, aroma-related enzyme activities, and gene expression of oriental melon with urea treatment as control under the same amount of nitrogen. The oriental melon cultivar “DX108” was used as experimental material. Aroma compositions and aroma-related enzyme activities in oriental melon fruits were investigated, and the expression levels of related genes from melon fruits under the different treatments, including alcohol dehydrogenase (ADH) and alcohol acyltransferase (AAT), were analyzed. The results showed that there were significant differences in the contents of aromatic compounds and aroma-related enzyme activities, and gene expression of oriental melon fruits. Urea treatment promoted the syntheses of C6 and C9 alcohols and aldehydes in melon fruit, however, the kinds and content of esters were significantly increased by chicken manure and chicken manure co-application with urea treatment, especially acetate esters. Furthermore, the content of non-acetate esters in fruits of chicken manure co-application with urea treatment was 3.1 times higher than that in fruits of urea treatment at day 35 after anthesis, and some characteristic esters were synthesized in fruits under the co-application treatment and chicken manure treatment, which were not observed in fruits from urea treatment. Both chicken manure and the co-application treatments increased the activities of lipoxygenase (LOX), aminotransferase, ADH, and AAT in fruits from day 25 to 30 during the development stage but reduced the activities of ADH in fruits at day 35 after anthesis. Meanwhile, the expressions of CmADH1 and CmADH2 in fruits with chicken manure and the co-application were suppressed, nevertheless the expressions of CmAAT1 and CmAAT3 were increased after day 30 during the development stage. In summary, either chicken manure or chicken manure co-applied with urea treatments may regulate the coordinated changes of aroma-related enzyme activities and gene expression of the aroma synthesis pathway of fruits at different developmental stages, which further affects the synthesis of fruit aroma substances, especially the synthesis of esters. |
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