| Effects of exogenous ATP on seed germination and seedling growth of Brassica campestris under salt stress |
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| DOI：10.7606/j.issn.1000-7601.2020.03.12 |
| Key Words: Brassica campestris seed soaking with exogenous ATP NaCl stress seed germination stress resistance physiology gene expression |
| Author Name | Affiliation | | LIANG Juanhong | School of Life Sciences, Northwest Normal University, Lanzhou, Gansu 730070, China | | LI Qiaoli | School of Life Sciences, Northwest Normal University, Lanzhou, Gansu 730070, China | | LAI Jing | School of Life Sciences, Northwest Normal University, Lanzhou, Gansu 730070, China | | ZHANG Xiaohua | School of Life Sciences, Northwest Normal University, Lanzhou, Gansu 730070, China | | ZHANG tengguo | School of Life Sciences, Northwest Normal University, Lanzhou, Gansu 730070, China |
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| Abstract: |
| Using Brassica campestris “Longyou 8” as test material, effects of exogenous ATP pretreatment on the seed germination and seedling biomass, Hsub>2O2 and ·OH content, antioxidant enzymes (CAT and POD) activities, membrane damage, osmoregulatory substances and expression of related genes (MAPK[STBX]3 and MAPK6, SOS1 and NHX1) [STBZ]of Brassica campestris were studied. The results showed that compared with the control (without ATP or NaCl treatment), the germination rate and germination energy of Brassica campestris seeds were increased to different degrees with different concentrations (1, 10, 25, 50 μmol ·L-1 and 100 μmol ·L-1) of exogenous ATP. The exogenous ATP treatment of 25 μmol·L-1 was particularly significant, and the germination rate and germination potential increased by 26.9% and 17.2%, respectively. Compared with NaCl stress treatment, 25 μmol·L-1 exogenous ATP + NaCl treatment increased the germination rate and germination energy of Brassica campestris seeds by 13.7% and 15.0%, respectively; significantly increased the biomass (root length, plant height and fresh weight) of Brassica campestris seedling, two antioxidant enzyme activities (CAT、POD), soluble sugar and proline content and related gene ([STBX]MAPK3 and MAPK6, SOS1 and NHX1)[STBZ] amount of express; increased root length, plant height, and fresh weight by 23.6%, 27.3% and 28.6%, respectively. Also, the activities of CAT, POD, soluble sugar, and proline increased by 10.3%, 27.0%, 15.9%， and 41.0%, respectively, while the contents of H2O2 and ·OH, malondialdehyde (MDA), and relative conductivity significantly decreased by 13.6%, 6.3%, 27.6%, and 20.5%, respectively. The results indicated that exogenous ATP soaking promoted the seed germination of Brassica campestris under NaCl stress, significantly increased the biomass, antioxidant capacity and expression of related salt\|tolerant genes, reduced its membrane damage and enhanced the salt tolerance of Brassica campestris seedlings. |
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