| Effects of subsurface drip irrigation with micro\|nano bubbled water on soil enzyme activity and root prolineof alfalfa |
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| DOI：10.7606/j.issn.1000-7601.2020.04.09 |
| Key Words: subsurface drip irrigation micro\|nano bubbled water alfalfa soil enzyme activity root characteristics root proline content |
| Author Name | Affiliation | | CAO Xuesong | Institute of Water Resources for Pastoral Area of China Institute of Water Resources and Hydropower Research, Huhhot, Inner Mongolia 010020, China | | ZHENG Hexiang | Institute of Water Resources for Pastoral Area of China Institute of Water Resources and Hydropower Research, Huhhot, Inner Mongolia 010020, China | | TONG Changfu | Institute of Water Resources for Pastoral Area of China Institute of Water Resources and Hydropower Research, Huhhot, Inner Mongolia 010020, China | | LI Heping | Institute of Water Resources for Pastoral Area of China Institute of Water Resources and Hydropower Research, Huhhot, Inner Mongolia 010020, China | | LIU Ruichun | Office of Flood Control and Drought Relief in Hangjinqi, Ordos City, Inner Mongolia Autonomous Region, Ordos, Inner Mongolia 017400， China | | HAO Yuxin | Mengcheng Construction of the Limited Company, Huhhot, Inner Mongolia 010020, China |
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| Abstract: |
| In order to explore the effects of different dissolved oxygen levels on soil enzyme activity and root proline of alfalfa under the condition of subsurface drip irrigation with micro\|nano bubbled water, field trials were used with the same irrigation quota. Three dissolved oxygen mass concentration levels of micro\|nano bubbled water (low: 1.8 mg·L-1, WA-1; medium: 5.0 mg·L-1, WA-2; and high: 8.2 mg·L-1, WA-3) and unfilled conventional irrigation treatment (CK) were set to study the status of catalase, urease activity, root characteristics, and free proline content in roots of alfalfa rhizosphere under different treatments. The results showed that subsurface drip irrigation with micro\|nano bubbled water significantly increased the activity of soil catalase and urease in the root zone of alfalfa.During the period, with increasing the dissolved oxygen mass concentration of micro\|nano bubbled water, the activities of soil catalase and urease showed an increasing trend. Under the same experimental treatment conditions, the activities of soil catalase and urease showed a trend of rising first and then decreasing with increasing the number of subsurface drip irrigation with micro\|nano bubbled water. The maximum soil catalase and urease activities of WA-3, WA-2, and WA-1 were 20.28, 19.19, 16.92 ml·g-1 and 13.98, 13.17, 12.07 mg·g-1·d-1, respectively, equivalent to increases of 46.74%, 38.86%, 22.43% and 45.17%, 36.76%, 25.34%, respectively, compared with 13.82 ml·g-1 and 9.63 mg·g-1·d-1for CK. Micro\|nano bubbled water subsurface drip irrigation reduced the free proline content of roots and treatment with WA-2 had the lowest free proline content at 51.01 μmol·g-1, which was a decrease by 44.55% compared with 92 μmol·g-1 for CK. This shows that subsurface drip irrigation with micro\|nano bubbled water alleviated the phenomenon of weakened soil permeability caused by long\|term subsurface drip irrigation, improved soil oxygen diffusion rate, enhanced soil enzyme activity, promoted aerobic respiration of root system and the growth and development of the whole plant. |
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