| Cloning and functional analysis of potato stomatal density epidermal patterning factor StSTOMAGEN |
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| DOI：10.7606/j.issn.1000-7601.2020.04.30 |
| Key Words: stomatal density StSTOMAGEN drought tolerance potato |
| Author Name | Affiliation | | WANG Yanli | College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China | | XIE Tian | College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China | | ZHANG Chunli | College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China | | LI Juanjuan | College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China | | LIU Yijian | Institute of Soil and Water Conservation, Northwest A&F University, Yangling, Shaanxi 712100, China | | LI Hongbing | Institute of Soil and Water Conservation, Northwest A&F University, Yangling, Shaanxi 712100, China | | YIN Li’na | Institute of Soil and Water Conservation, Northwest A&F University, Yangling, Shaanxi 712100, China | | WANG Shiwen | Institute of Soil and Water Conservation, Northwest A&F University, Yangling, Shaanxi 712100, China | | DENG Xiping | College of Life Sciences, Northwest A&F University, Yangling, Shaanxi 712100, China
Institute of Soil and Water Conservation, Northwest A&F University, Yangling, Shaanxi 712100, China | | KE Qingbo | Institute of Soil and Water Conservation, Northwest A&F University, Yangling, Shaanxi 712100, China |
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| Abstract: |
| Secretion of small molecules in the epidermal patterning factor\|like family (EPFL) play an important role in stomatal development. To study the function of potato EPFL9/StSTOMAGEN gene during stomatal development, we cloned and functionally characterized the stomatal density regulator StSTOMAGEN from potato (Atlantic) and transformed it into Arabidopsis by Agrobacterium\|mediated genetic transformation. We found StSTOMAGEN was mainly inter cellular space and cell nucleus localized and had the highest expression level in the apical unexpanded leaves. Interestingly, StSTOMAGEN transcript was rhythmically expressed with a peak around Zeitgeber time (ZT) 20 under long\|day (16 h/8 h, light/dark) conditions. Expression of StSTOMAGEN was markedly down\|regulated in response to abscisic acid (ABA) and sodium chloride (NaCl) treatments; however, upon the application of polyethylene glycol (PEG), the expression of StSTOMAGEN peaked at 4 h and then decreased gradually. Functional identification showed that the stomatal density of the StSTOMAGEN overexpression (ST) lines was significantly increased by 63%~83%, and led to elevated photosynthetic rateby 36%~42%. Moreover, ST lines showed more water loss (24%~55%) and hydrogen peroxide (H2O2) accumulation (50%~100%) in detached leaves compared with wild\|type (WT) plants. Under drought stress, ST lines maintained lower photosynthetic rates (55%~66%), photosystem Ⅱ efficiency (22%~50%), and instantaneous water use efficiency (111%~12%) than WT plants did. Taken together, the results showed that the epidermal patterning factor StSTOMAGEN reduced drought tolerance of plants by positively regulating stomatal density. This result laid a theoretical foundation for reducing the expression of StSTOMAGEN gene and cultivating drought\|resistant and water\|saving potato strains by gene editing technology in the later period. |
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