| Effects of years of alfalfa planting on nitrification potential and abundance of soil ammonia oxidation microorganisms |
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| DOI：10.7606/j.issn.1000-7601.2020.05.06 |
| Key Words: alfalfa years of planting ammonia\|oxidizing microorganisms abundance nitrification potential soil nitrogen |
| Author Name | Affiliation | | ZHANG Yaoquan | Resource and Environmental Faculty of Gansu Agricultural University, Lanzhou, Gansu 730070, China | | MA Xin | Resource and Environmental Faculty of Gansu Agricultural University, Lanzhou, Gansu 730070, China | | LUO Zhuzhu | Resource and Environmental Faculty of Gansu Agricultural University, Lanzhou, Gansu 730070, China
Gansu Key Laboratory of Aridland Crop Science, Lanzhou, Gansu 730070, China | | NIU Yining | Gansu Key Laboratory of Aridland Crop Science, Lanzhou, Gansu 730070, China | | LI Lingling | Gansu Key Laboratory of Aridland Crop Science, Lanzhou, Gansu 730070, China | | CAI Liqun | Resource and Environmental Faculty of Gansu Agricultural University, Lanzhou, Gansu 730070, China
Gansu Key Laboratory of Aridland Crop Science, Lanzhou, Gansu 730070, China | | XIE Junhong | Gansu Key Laboratory of Aridland Crop Science, Lanzhou, Gansu 730070, China |
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| Abstract: |
| This experiment relied on the alfalfa (Medicago sativa) cultivation with different planting years (L2003, L2005, and L2012) on Loess Plateau. We investigated the soil nitrate N, ammonium N, nitrification potential, the abundance of ammonia oxidization Archaea（AOA）and ammonia oxidization Bacteria (AOB), and analyzed the effects of physicochemical indexes on the abundance of ammonia\|oxidizing microorganisms. The results showed that the nitrification potential of soil gradually decreased with increasing years of alfalfa planting and different years of planting had significant differences (P<0.05), which nitrification potentials of L2012, L2005 and L2003 were 0.05, 0.04 μg·g-1·h-1 and 0.04 μg·g-1·h-1, respectively. Soil total N gradually significantly increased with increasing years of alfalfa planting (P<0.05), which total nitrogen contents of L2012, L2005 and L2003 were 1.02, 1.14 g·kg-1 and 1.18 g·kg-1, respectively. The total N contents of L2003 and L2005 were higher than L2012 by 15.69% and 11.76%, respectively. Soil nitrate N significantly increased gradually with increasing years of alfalfa planting (P<0.05). The soil nitrate N contents of L2003 and L2005 were higher than that of L2012 by 59.73% and 33.62%, respectively. The number of copies of AOA amoA gene was 9.75×106~12.68×106·g-1， which was significantly higher than 5.01×106~7.70×106·g-1 of AOB, and the AOA abundance gradually increased with increasing the years of alfalfa planting significantly. The AOB abundance showed a significant trend of increasing first and then decreasing with increasing years of alfalfa planting (P<0.01). This indicates that AOA was the dominant microorganism of ammonia oxidation in alfalfa soil in semi\|arid area of loess plateau. The correlation analysis showed that AOA abundance was significantly positively correlated with total N (r=0.853) and nitrate N (r=0.833) but significantly negatively correlated with nitrification potential (r=-0.802), and AOB abundance was not significantly correlated with physicochemical properties. The results also showed that AOA was the dominant group of ammonia\|oxidizing microorganisms in alfalfa soil in the semi\|arid area of the Loess Plateau, which had a stronger response to alfalfa growth years, but it did not lead the soil nitrification process. |
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