| Screening of highly effective biocontrol Bacillus strains for potato scab and optimization of its fermentation conditions |
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| DOI：10.7606/j.issn.1000-7601.2020.05.36 |
| Key Words: biocontrol Bacillus atrophy inhibition potato scab lipopeptide antibiotics optimization of fermentation conditions |
| Author Name | Affiliation | | ZHOU Yangzi | College of Resources and Environmental Sciences, Gansu Provincial Key Lab of Aridland Crop Science, Gansu Agricultural University, Lanzhou, Gansu 730070
Gansu Provincial Livestock and Poultry Waste Resource Utilization and Engineering Center, Lanzhou, Gansu 730070 | | QIU Huizhen | College of Resources and Environmental Sciences, Gansu Provincial Key Lab of Aridland Crop Science, Gansu Agricultural University, Lanzhou, Gansu 730070
Gansu Provincial Livestock and Poultry Waste Resource Utilization and Engineering Center, Lanzhou, Gansu 730070 | | DONG Li | College of Resources and Environmental Sciences, Gansu Provincial Key Lab of Aridland Crop Science, Gansu Agricultural University, Lanzhou, Gansu 730070
Gansu Provincial Livestock and Poultry Waste Resource Utilization and Engineering Center, Lanzhou, Gansu 730070 | | WEI Ruyun | College of Resources and Environmental Sciences, Gansu Provincial Key Lab of Aridland Crop Science, Gansu Agricultural University, Lanzhou, Gansu 730070
Gansu Provincial Livestock and Poultry Waste Resource Utilization and Engineering Center, Lanzhou, Gansu 730070 | | DONG Aiju | College of Resources and Environmental Sciences, Gansu Provincial Key Lab of Aridland Crop Science, Gansu Agricultural University, Lanzhou, Gansu 730070
Gansu Provincial Livestock and Poultry Waste Resource Utilization and Engineering Center, Lanzhou, Gansu 730070 | | WANG Youling | College of Resources and Environmental Sciences, Gansu Provincial Key Lab of Aridland Crop Science, Gansu Agricultural University, Lanzhou, Gansu 730070
Gansu Provincial Livestock and Poultry Waste Resource Utilization and Engineering Center, Lanzhou, Gansu 730070 | | WANG Chuan | College of Veterinary Medicine, Gansu Agricultural University, Lanzhou, Gansu 730070 |
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| Abstract: |
| The incidence of potato scab caused by Streptomyces scabies is increasing in the main potato production regions in Gansu Province. It is urgent to explore safe and effective biological control methods for this disease. This experiment screened the inhibitory effects of the identified 5 strains of Bacillus on potato scab pathogens, which were significantly antagonistic to potato black mole disease pathogens, the biocontrol mechanism was studied by detecting the genes related to the synthesis of biocontrol lipopeptide antibiotics. The results of the confrontation experiment showed that among the five strains of biocontrol bacteria, QHZ-3 had the best inhibitory effect on the pathogen of potato scab, and the inhibition rate was 44%. Antibiotic synthesis gene test results showed that QHZ-1 and QHZ-2 had fengycin and iturin synthase genes. The QHZ-4 had only fengycin synthase genes, and QHZ-3 and QHZ-5 strains had three lipopeptide antibiotic synthetase genes of surfactin gene, iturin gene and fengycin gene. The QHZ-3 with the most biocontrol potential was selected for further study, and it was found that the diameter of the inhibition zone of the fermentation filtrate on Streptomyces scabies reached 30.4 mm, and under the conditions of high temperature, ultraviolet radiation and strong acid and alkali, the diameter of the bacteriostatic circle was still kept above 27 mm. The results of single\|factor experiments showed that the growth rate and antibacterial activity of strain QHZ-3 were the highest under the conditions of 60 mL pre 250 mL triangular flask, 6% inoculation volume, 180 r·min-1 shaker speed, and 60 h fermentation time. Orthogonal experiment results showed that under the combination condition of 40 mL per triangle flask, shaker speed of 170 r·min-1, inoculation amount of 6%, and fermentation time of 48 h, QHZ-3 had the best growth state and the number of viable bacteria reached 1.31×1010 cfu·mL-1. The results of the optimization of the secondary solid fermentation conditions showed that use the composted cow manure organic fertilizer as the carrier, the number of viable bacteria was the highest when the inoculation amount was 15%, reaching 1.1×109 cfu·mL-1. |
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