| Effects of drought\|tolerance Rhizobium on the Dolichos lablab growth under drought stress |
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| DOI：10.7606/j.issn.1000-7601.2020.06.06 |
| Key Words: drought\|tolerance Rhizobium promoting plant growth Dolichos lablab L. drought stress physiological index degraded rocky desert habitats |
| Author Name | Affiliation | | ZHANG Shuqing | School of Geography and Resources, Guizhou Normal University, Guiyang, Guizhou 550018, China
Institute of Soil and Environment Bioremediation in Karst Habitats, Guizhou Normal University, Guiyang, Guizhou 550018, China | | ZANG Dandan | School of Geography and Resources, Guizhou Normal University, Guiyang, Guizhou 550018, China | | LI Jianfeng | Institute of Soil and Environment Bioremediation in Karst Habitats, Guizhou Normal University, Guiyang, Guizhou 550018, China | | GUO Jinmei | School of Geography and Resources, Guizhou Normal University, Guiyang, Guizhou 550018, China
Institute of Soil and Environment Bioremediation in Karst Habitats, Guizhou Normal University, Guiyang, Guizhou 550018, China |
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| Abstract: |
| To investigate the effects of drought\|tolerant Rhizobium on the growth of Dolichos lablab seedlings under drought stress, a sand\|culture experiment was conducted to evaluate the effect of Rhizobium radiobacter Y-1 (Y-1), Rhizobium radiobacter 4-4 (4-4), and Rhizobium radiobacter 37-1 (37-1) on seedlings of Dolichos lablab under drought\|stress condition. Surface sterilized and germinated seeds of Dolichos lablab cv. Rongai were sown in Plastics pots filled with clear sand at the top and rock debris (from rocky desertification area) at the bottom. The pots of each treatment were inoculated with sterilized water (control) or Rhizobium solution of Y-1, 4-4, or 37-1. Calculated and supplied the sterilized water to simulate the mild, moderate, and severe drought stress in severe rocky desertification habitat. Results showed that the seedlings under drought stress were significantly promoted when seeds were inoculated with Rhizobium radiobacter Y-1 or Rhizobium radiobacter 37-1. On day 30, all of growth attributes and physiology of seedlings were significantly better than that of the control (inoculated with sterilized water) under drought stress. Under severe drought stress (30% of field water capacity, θf), the plant height, leaf area, root length, root fresh weight and root volume of seedlings inoculated with Y-1 increased by 50.25%, 73.17%, 72.41%, 136.00%, and 289.47%, respectively, compared with those of the control. Plant soluble protein content increased by 108.57% and maximum quantum efficiency of PSⅡ photochemistry (Fv/Fm) increased by 42.09% compared with that of the control while leaf MDA content of Y-1 treatment was only 60.66% of the control (P<0.05). Under mild and moderate drought stress (45%~60% of θf), plant root length of 37-1 treatment was 39.93%~47.99% of that of the control while MDA content of 37-1 treatment was only 72.14%~90.92% of that of the control. Inoculated with drought\|tolerant Rhizobium radiobacter Y-1 and Rhizobium radiobacter 37-1 accelerated root growth, root volume, leaf area, and the formation of soluble protein, decreased leaf MDA content, and increased the root activity and the leaf maximum quantum efficiency of PSⅡ photochemistry under the drought stress, indicating that as a kind of cultivation method, the seed inoculated with drought\|tolerant Rhizobium promoted the seedling growth and resistance under drought stress conditions. |
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