| Effect of exogenous hydrogen sulfide on seed germination and seedling growth in naked oat under osmotic stress |
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| DOI：10.7606/j.issn.1000-7601.2020.06.09 |
| Key Words: hydrogen sulfide(H2S) naked oat osmotic stress seed germination seedling growth |
| Author Name | Affiliation | | LIU Jianxin | University Provincial Key Laboratory for Protection and Utilization of Longdong Bio\|resources in Gansu Province/College of Life Sciences and Technology, Longdong University, Qingyang, Gansu 745000, China | | LIU Ruirui | University Provincial Key Laboratory for Protection and Utilization of Longdong Bio\|resources in Gansu Province/College of Life Sciences and Technology, Longdong University, Qingyang, Gansu 745000, China | | JIA Haiyan | University Provincial Key Laboratory for Protection and Utilization of Longdong Bio\|resources in Gansu Province/College of Life Sciences and Technology, Longdong University, Qingyang, Gansu 745000, China | | BU Ting | University Provincial Key Laboratory for Protection and Utilization of Longdong Bio\|resources in Gansu Province/College of Life Sciences and Technology, Longdong University, Qingyang, Gansu 745000, China | | LI Na | University Provincial Key Laboratory for Protection and Utilization of Longdong Bio\|resources in Gansu Province/College of Life Sciences and Technology, Longdong University, Qingyang, Gansu 745000, China |
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| Abstract: |
| Hydrogen sulfide (H2S) is a novel type gas signaling molecule in plants. The effects of exogenous H2S on the seed germination and seedling growth of naked oat (Avena nude) under osmotic stress were investigated．Seeds of naked oat cultivar ‘Dingyou 9’ were used as materials for a two\|factor experiment including concentration of sodium hydrosulfide (NaHS, a donor of H2S) and osmotic stress. Four different concentrations of NaHS (0, 0.2, 1.0, and 5.0 mmol·L-1) were set to soak the seeds for 8 hours, and osmotic stress was simulated using five different concentrations of polyethylene glycol (PEG-6000) (0, 119, 149, 178 g·L-1, and 202 g·L-1). A total of 20 treatments were conducted to determine germination characteristics (germination potential, germination rate, germination index, vigor index, mean germination time, and storage material transformation rate) and seedlings growth parameters (radicle length, plumule length, and seedling dry weight). Subordinate function analysis was also carried out for comprehensive evaluation of osmotic tolerance of naked oat seeds in different treatments. The results showed that: (1) The NaHS concentration and osmotic stress had significant effects on the parameters of seed germination and seedling growth of naked oat. The interaction between them had significant effects on germination index, vigor index, mean germination time, and radicle length. (2) With the enhancement of osmotic stress, which was simulated by PEG, the all parameters of seed germination and seedling growth decreased significantly under the same NaHS concentration, but only the average germination time increased dramatically. (3) At the same PEG concentration, soaking seeds with 0.2 mmol·L-1 NaHS had no significant effect on the parameters of seed germination and seedling growth of naked oats. Soaking seeds with 1.0 mmol·L-1 NaHS increased germination index, vigor index, storage material transformation rate, radicle length, plumule length, and seedling dry weight, while decreased average germination time. Soaking seeds with 5.0 mmol·L-1 NaHS increased the mean germination time, while all other seed germination and seedling growth parameters showed a decreasing trend. (4) The result of subordinate function analysis showed that the soaking seeds with 1.0 mmol·L-1 NaHS improved the comprehensive evaluation value (D) under osmotic stress simulated by PEG, while the D value of soaking seeds with 5.0 mmol·L-1 NaHS significantly decreased. These results indicated that soaking seeds with appropriate concentration of NaHS (1.0 mmol·L-1) can effectively improve the seed vigor of naked oats and promote its seed germination and seedling growth under osmotic stress. |
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